| Construction of Antisense Transforming Growth Factorβ_1 Gene and Its Effect on the Proliferation by Expression in Osteosarcoma Cells |
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| 引用本文： | 刘勇,郑启新,杜靖远,杨述华,邵增务,肖宝钧. Construction of Antisense Transforming Growth Factorβ_1 Gene and Its Effect on the Proliferation by Expression in Osteosarcoma Cells[J]. 华中科技大学学报(医学英德文版), 2003, 23(2) |
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| 作者姓名： | 刘勇 郑启新 杜靖远 杨述华 邵增务 肖宝钧 |
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| 作者单位： | Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，Department of Orthopaedics，Xiehe Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022 |
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| 基金项目： | This work was supported by a grant from Chenguang Project of Wuhan(Serial No.20025001028). |
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| 摘 要： | Summary:To construct the antisense transforming growth factorβ1(TGFβ1)gene and investigatethe effect of TGFβ1 autocrine loop blockage on the proliferation of osteosarcoma cells.TGFβ1 cDNAwas cloned by RT-PCR from human osteosarcoma cells(MG-63)and inserted into pcDNA_3 to con-struct an antisense expression vector,which was dubbed pcDNA_3-TGFβ1(-).MTT was used to de-tect the proliferation of osteosarcoma cells transfected by antisense TGFβ1 gene.Our results showedthat the proliferation of the transfected osteosarcoma cells was suppressed markedly.It is concludedthat TGFβ1 autocrine loop blockage in osteosarcoma cells could inhibit cell proliferation,which mightbe helpful for gene therapy of osteosarcoma.
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| 关 键 词： | transfoming growth factorβ1 autocrine loop osteosarcoma transfection |
Construction of Antisense Transforming Growth Factorβ1 Gene and Its Effect on the Proliferation by Expression in Osteosarcoma Cells |
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| LIU Yong ZHENG Qixin DU Jingyuan YANG Shuhua SHAO Zengwu XIAO Baojun. Construction of Antisense Transforming Growth Factorβ1 Gene and Its Effect on the Proliferation by Expression in Osteosarcoma Cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2003, 23(2) |
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| Authors: | LIU Yong ZHENG Qixin DU Jingyuan YANG Shuhua SHAO Zengwu XIAO Baojun |
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| Affiliation: | Department of Orthopaedics, X iehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 |
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| Abstract: | Summary: To construct the antisensc transforming growth factorβl (TGFβ1) gene and investigatethe effect of TGFβ1 autocrine loop blockage on the proliferation of osteosarcoma cells. TGFβ1 cDNAwas cloned by RT-PCR from human osteosarcoma cells (MG-63) and inserted into pcDNA3 to con-struct an antisense expression vector, which was dubbed pcDNA3-TGFβ1(- ). MTT was used to de-tect the proliferation of osteosarcoma cells transfected by antisense TGFβ1 gene. Our results showedthat the proliferation of the transfected osteosarcoma cells was suppressed markedly. It is concludedthat TGFβ1 autocrine loop blockage in osteosarcoma cells could inhibit cell proliferation, which mightbe helpful for gene therapy of osteosarcoma. |
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| Keywords: | autocrine loop osteosarcoma transfection |
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