人参皂甙对CD34+造血干/祖细胞的增殖和分化作用

目的：探索人参皂甙（ＧＳ）对ＣＤ３４＾＋造血干／祖细胞的刺激增殖和诱导分化作用。方法：采用免疫磁珠法（ＭＡＣＳ）分离纯化脐血ＣＤ３４＾＋细胞，在造血细胞半固体和液体培养体系中加入不同浓度的ＧＳ，检测对ＣＤ３４＾＋造血干／祖细胞增殖，形成祖细胞集落的提高率，并用流式细胞仪检测液体培养后细胞表面标记的变化。结果：ＧＳ（５～５０μｇ／ｍｌ）能提高ＢＦＵ－Ｅ、ＣＦＵ－Ｅ、ＣＦＵ－ＧＭ、ＣＦＵ－ＧＥＭＭ集落

Effect of Ginsenosides on Proliferation and Differentiation of Human CD34+ Hematopoietic Stem/Progenitor Cells

OBJECTIVE: To investigate the effect of ginsenosides (GS) on proliferation and differentiation of human CD34+ stem/progenitor cells. METHODS: CD34+ hematopoietic progenitor cells were isolated from umbilical cord blood by using the immune beads sorting system. The cells were exposed to GS of different concentrations in both liquid culture and semi-solid culture, and the elevation rate on proliferation of CD34+ stem/progenitor cells and colony formation were estimated. The cells were marked with monoclonal antibody and the marker was examined by flow cytometry after incubated with GS for 14 days. RESULTS: GS (5-50 micrograms/ml) could raise the colony production rate of BFU-E, CFU-E, CFU-GM, CFU-GEMM by (87.6 +/- 2.6)%, (63.3 +/- 2.8)%, (58.0 +/- 3.1)% and (96.3 +/- 5.5)% respectively (all P < 0.01), and the best effect in improving cell proliferation of CD34+ cells in vitro was obtained when the concentration of GS was 25 micrograms/ml. After incubation with GS for 14 days, number of CD33+ cells was increased by GS in a dose-dependent manner with a peak increasing rate at 200 micrograms/ml. In the presence of GS 50 micrograms/ml, CD15+ cells were reaching the peak. Number of CD71+ and G-A+ cells increased only when the concentration of GS was 25 micrograms/ml. CONCLUSION: GS could not only promote the proliferation but also induce the differentiation of CD34+ hematopoietic stem/progenitor cells, GS may play the role by cooperating with hematopoietic growth factor, and by its growth factor-like function in the regulation of hematopoiesis.