Characterization of the rough endoplasmic reticulum ribosome-binding activity. |
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Authors: | J M Nunnari D L Zimmerman S C Ogg P Walter |
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Affiliation: | Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143-0448. |
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Abstract: | The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein. |
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