不同途径移植间充质干细胞对大鼠急性肝损伤的修复作用

目的:评价骨髓间充质干细胞(MSCs)移植对大鼠急性肝损伤的修复作用,探讨其可行性和有效性.方法:四氯化碳(CCl4)腹腔注射复制Wistar大鼠急性肝损伤模型,造模后随机分为实验组及对照组,实验组分别通过尾静脉、门静脉、肝内注射移植经Hochest33342标记的大鼠骨髓MSCs悬液0.5 mL(约1×10 6个细胞...

Repair effect of mensenchymal stem cells transplantation by different routes on acute liver injury of rats

Objective To evaluate the repair effect of bone marrow mensenchymal stem cells(MSCs) transplantation on acute liver injury of rats and to explore the feasibility and validity.Methods The Wistar rats were treated with 10% CCl4 olive oil mixture by intraperitoneal injection to copy acute liver injury model.The model rats were randomly divided into treatment and control groups.Hochest33342-labelled rat bone marrow MSCs 0.5 mL(1×106 cells) were transplanted into the rats by tail vein,portal vein and liver injection in treament groups,respectively.The same volume PBS was transfused into the rats in various control groups.On day 3 and 7 after transplantation,the liver function indexes of rats were detected,the distribution of Hochest33342-labelled cells and the pathological changes of liver tissue were observed by fluorescence microscopy at same time.Results Compared with control group,the levels of serum ALT and AST were decreased on day 3 and day 7 after transplantation in intraportal vein and intraliver injection groups(P<0.01),but there were no statistically significant differences in tail vein group(P>0.05);and they were decreased in intraliver injection group compared with intraportal vein group(P<0.05).The Hochest33342 labelled cells were not found in vein group and control groups at different time,but they were found in intraportal vein and intraliver injection groups;the days were more,the cells were more.The pathological structure of liver displayed multiple necrosis of hepatocyte,diffuse degeneration of hepatocyte and infiltration of inflammatory cells in rats treated with CCl4 for 24h.The severity of pathological structure of liver wasn’t alleviated in tail vein group at different time after transplantation,but it was alleviated in intraportal vein and intraliver injection groups on day 3 and day 7;the improvement of pathohistology intraliver injection group was better than that in intraportal vein group.Conclusion Intraportal vein,intraliver injection transplantation of bone marrow MSCs can ameliorate and repair liver function of rats with acute liver injury.The route of intraliver injection transplantation is better than intraportal vein transplantation.