胃癌组织p53、p16基因表达的研究

目的探讨胃癌p53、p16基因表达与临床病理因素的关系.方法采用免疫组化SP法检测63例胃癌患者的原发灶、转移淋巴结及正常粘膜组织的p53、p16基因表达情况,分析其与临床病理因素之间的关系.结果p53基因与胃癌淋巴转移密切相关,P16基因表达与胃癌临床病理诸因素无关,p53高表达与p16低表达联合与淋巴结转移有关.结论p53基因的阳性表达与胃癌恶性程度,特别是与淋巴结转移有关,可以作为胃癌淋巴结转移的标志物.

Immunohistochemical analysis of p53 and p16 genes expression in human stomach cancer

Objective To investigate the relationship between p53 and p16 genes with clinical pathological factors of patients with stomach cancer. Methods Expression of p53 and p16 genes in specimens of primary tumor, metastasis lymph nodes and normal membrane of 63 patients with gastric cancer were immunohistochemically stained, and the relationship between the gene expression and clinical pathological factors was analyzed. Results The overexpression of p53 gene was correlated with the lymph nodes metastasis and was not relative with other clinic pathological factors. The lowexpression of p16 gene was not relative with all the factors. Conclusion The p53 genes overexpression would be a good marker for human gastric cancer, it is relative with lymph node metastasis.