| 类风湿关节炎患者血清IL-35的水平及其与疾病活动度的关系 |
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| 引用本文： | 孟博,谢而付,周春刚.类风湿关节炎患者血清IL-35的水平及其与疾病活动度的关系[J].临床检验杂志,2016(4):277-280. |
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| 作者姓名： | 孟博 谢而付 周春刚 |
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| 作者单位： | 南京中医药大学附属无锡中医院检验科，江苏无锡 214071,南京医科大学第一附属医院检验学部，南京 210029,南京中医药大学附属无锡中医院中心实验室，江苏无锡 214071 |
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| 摘 要： | 目的探讨类风湿关节炎(RA)患者血清IL-35表达水平与疾病活动度的相关性。方法选取确诊RA患者118例,分为活动期组62例和缓解期组56例。同时选取90例健康人作为对照,ELISA法检测RA患者及对照者血清IL-35水平,实时荧光定量PCR检测外周血单个核细胞(PBMC)中IL-35亚基EBI3和P35 mRNA表达水平。分析血清IL-35水平与DAS28评分、C反应蛋白(CRP)、红细胞沉降率(ESR)、类风湿因子(RF)、关节肿胀数(SJC)和关节压痛数(TJC)等的相关性。结果活动期组RA患者血清中IL-35浓度(38.09±16.90)pg/m L显著低于对照组(82.25±15.82)pg/m L,P0.01)]和缓解期组(72.96±11.74)pg/m L,P0.01)];依据DAS28评分将活动期组RA患者分为轻度、中度和重度组,血清IL-35水平(pg/m L)在3组中依次降低(轻度60.95±7.31,中度39.68±9.51,重度28.02±9.16),轻度组与中度或重度组间差异具有统计学意义(P0.05)。活动期组和缓解期组患者血清IL-35水平与SJC、CRP和DAS28评分呈负相关性(P0.05),与ESR、TJC、RF无相关性。PBMC中P35 mRNA在RA活动期组中显著降低,低于健康对照组和缓解期组(0.47±0.18 vs 1.91±1.121.82±0.53,P0.01),但EBI3 mRNA在活动期组(1.62±0.68)、缓解期组(1.48±0.77)及对照组(1.86±1.21)中差异无统计学意义(P0.05)。结论活动期组RA患者血清IL-35水平显著下降,且伴随P35 mRNA水平下降,并与RA疾病活动度密切相关,可作为RA疾病活动度评价的潜在指标。
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| 关 键 词： | 类风湿关节炎 IL-35 DAS28 EBI3 P35 |
| 收稿时间： | 2016/1/17 0:00:00 |
The levels of serum interleukin 35 in patients with rheumatoid arthritis and its correlation with disease activity |
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| Meng Bo;Xie Er-fu;Zhou Chun-gang.The levels of serum interleukin 35 in patients with rheumatoid arthritis and its correlation with disease activity[J].Chinese Journal of Clinical Laboratory Science,2016(4):277-280. |
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| Authors: | Meng Bo;Xie Er-fu;Zhou Chun-gang |
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| Affiliation: | Meng Bo;Xie Er-fu;Zhou Chun-gang;Department of Clinical Laboratory,Wuxi Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine;Department of Laboratory Medicine,The First Affiliated Hospital of Nanjing Medical University;Central Laboratory,Wuxi Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine; |
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| Abstract: | |
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| Keywords: | rheumatoid arthritis interleukin 35 DAS28 score epstein barr virus induced 3 P35 |
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