Development of Novel Microsatellite DNA Markers from the Pacific Oyster Crassostrea gigas |
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Authors: | Masashi Sekino Masami Hamaguchi Futoshi Aranishi Kenji Okoshi |
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Affiliation: | (1) Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shinhama, Shiogama, Miyagi 985-0001, Japan;(2) Research Institute of Seto Inland Sea, Fisheries Research Agency, Maruishi, Ohno, Saeki, Hiroshima 739-0452, Japan;(3) National Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama 236-8648, Japan;(4) Department of Biotechnology, Shinmito, Ishinomaki Senshu University, Minamisakai, Ishinomaki, Miyagi 986-8580, Japan |
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Abstract: | We document the potential of novel microsatellites as a genetic tool in furthering our understanding of the Crassostrea gigas genetic structure. From the microsatellite-enriched libraries we constructed, 123 repeat regions that had sufficient sequence information to design polymerase chain reaction primer sets were isolated. From these, 9 primer pairs were screened in a C. gigas population of 67 individuals to evaluate the genetic variability. All but 1 of the 9 loci showed allelic variation (number of alleles, 2–20; observed heterozygosity, 0.119–0.925; unbiased expected heterozygosity, 0.139–0.914). Considerable discrepancy of genotypic proportions from the Hardy-Weinberg equilibrium was observed at 1 locus with an apparent heterozygote deficiency. Several loci were successfully amplified in 3 other related species with the appropriate allele size: 6 loci in C. sikamea, 4 loci in C. ariakensis, and 5 loci in C. nippona. |
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Keywords: | pacific oyster Crassostrea gigas microsatellites genetic variability |
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