吸烟致大鼠慢性支气管炎形成过程气道上皮ICAM-1表达的研究

目的 探讨大鼠慢性支气管炎形成过程的不同阶段，气道上皮细胞间黏附分子ICAM-1的表达情况。方法 混合烟雾吸人法建立慢性支气管炎形成的系列发展模型：SP法测定气道上皮细胞细胞间黏附分子ICAM-1的表达，并与病理形态学改变的半定量评分、支气管肺泡灌洗液中炎症细胞数及分类以及肺组织的髓过氧化物酶（MPO）活性进行相关性分析。结果 在慢性支气管炎形成过程中，气道上皮ICAM-1的表达呈逐渐上调趋势，与气道病理变化及肺组织MPO活性呈正相关（分别为P〈0．01，P〈0．05）。结论 大鼠慢性支气管炎形成过程中，ICAM-1为介导炎症的重要因子。

Study on ICAM-1 Expression on Bronchial Epithelial Cells of the Chronic Bronchitis Rat Model During the Inflammation Process

Objective To study the ICAM-1 expression on bronchial epithelial cells of the sequential smoke-induced chronic bronchitis rat model during the inflammation process.Methods Chronic bronchitis sequential rat model was established by passively inhaling smoke mixture.Experiments were performed on 30 young male Sprague-Dawley rats,which comprised 5 groups in random,i.e.4 chronic bronchitis model groups and 1 control group.The levels of ICAM-1 expression were determined by immunohistochemistry method.Specimens were studied by semi-quantitative method to evaluate the morphologic changes in various stages.Meanwhile,the inflammatory cells of the BALF and the activity of MPO were analyzed.Results During the process of the chronic bronchitis,the ICAM-1 expression was increasing as time went on,positively related with the pathologic score(P<0.01),and the activity of MPO(P<0.05).Conclusion ICAM-1 may play an important role in the inflammatory process of chronic bronchitis.