| 体外培养的兔虹膜色素上皮细胞吞噬功能及细胞连接的观察 |
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| 引用本文： | 王兆艳,惠延年. 体外培养的兔虹膜色素上皮细胞吞噬功能及细胞连接的观察[J]. 眼科学报, 2001, 17(4): 191-193 |
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| 作者姓名： | 王兆艳 惠延年 |
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| 作者单位： | 1. 解放军总院眼科,北京,100852
2. 第四军医大学西京医院眼科,西安,710032 |
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| 摘 要： | 目的；观察体外培养和虹膜色素上皮（iris pigment epithelium IPE）细胞是否具有吞噬功能及屏障作用。方法：将鸡红细胞悬液加入体外培养的IPE细胞内，计算其吞噬数量，电镜下观察IPE细胞间连接。结果：体外培养状态下，IPE细胞对鸡红细胞的吞噬率为5．4％，视网膜色素上皮（retinal pigment epithelium RPE)细胞为8．8％（P＜0．01）；IPE同RPE一样具有紧密连接结构。结论：兔IPE在体外培养状态下，具有吞噬功能及屏障作用。
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| 关 键 词： | 吞噬功能 细胞连接 虹膜色素上皮 体外培养 动物实验 RPE |
Observation of Phagocytosis and Cell Junction of Rabbit IPE in Vitro |
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| Zhaoyan Wang,Yannian Hui. Observation of Phagocytosis and Cell Junction of Rabbit IPE in Vitro[J]. Eye science, 2001, 17(4): 191-193 |
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| Authors: | Zhaoyan Wang Yannian Hui |
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| Affiliation: | Department of Ophthalmology, Jeneral Hospital of People's Liberation Army, Beijing 100852, China. |
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| Abstract: | Purpose ;To study the phagocytosis and cell junctions of iris pigment epithelium(IPE) invitro.Methods: Chicken red blood cells were added to culture medium of IPE, the numbers ofIPE phagocyting chick red blood cells were counted; cell junctions were observed withtransmission electron microscope.Results: The phagocytotic rates of IPE and retinal pigment epithelium (RPE) were5. 4% and 8. 8% respectively; there were various tight junctions in IPE.Conclusion: IPE possessd phagocytosis and barrier functions in vitro. Eye Science2001; 17: 191 ~ 193. |
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| Keywords: | phagocytosis cell junction iris pigment epithelium (IPE) |
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