粘红酵母胞内L-苯丙氨酸解氨酶释放的研究

分别考察了液氮冷冻法、超声破碎法、甘氨酸与丙氨酸化学渗透法，不同化学渗透剂TritonX-100、Tween80、Span20、溴代十六烷基吡啶，有机溶剂甲苯、脲及EDTA对粘红酵母（Rhodotorula glutinis）胞内苯丙氨酸解氨酶（即PAL酶）释放及活力的影响，比较发现超声破碎与TritonX-100联用酶释放效果显著，先加入10％Triton X-100渗透6h，再在800W超声破碎30min，蛋白释放率达76．7％。总酶活70．3％，为提取胞内PAL酶提供了一种新方法。

Study on the release of phenylalanine ammonia-lyase in the cell of Rhodotorula glutinis

The effects of freezing treatment by liquid nitrogen,ultrasonic disruption,chemical penetration by L-glycine and L-alanine,and some surfactants(TritonX-100,Tween80,Span20,Cetyl pyridine bromide,toluene,urea and EDTA) on the release of phenylalanine ammonia-lyase(PAL) in the cell of Rhodotorula glutinis were investigated in this article.The experiments indicated that the combination of ultrasonic disruption and TritonX-100 could release the PAL significantly.By penetrating with TritonX-100 for 6 h,then ultrasonic disruption under 800 W for 30 min,the release rate of protein reached 76.7%,the enzyme activity of PAL stabilized at 70.3% of the original total activity.The research provides a new method for the extraction of PAL in yeast cells.