| 桑叶多酚氧化酶的固定化及酶学性质研究 |
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| 引用本文： | 杨洋,黄涛,关红艳,吴小玉. 桑叶多酚氧化酶的固定化及酶学性质研究[J]. 食品工业科技, 2010, 0(2) |
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| 作者姓名： | 杨洋 黄涛 关红艳 吴小玉 |
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| 作者单位： | 1. 广西大学生命科学与技术学院,广西南宁,530004
2. 广西大学轻工与食品工程学院,广西南宁,530004 |
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| 摘 要： | 研究了桑叶多酚氧化酶(PPO)的动力学特性,结果表明,其最适温度为40℃,最适pH为7.0,酶动力学参数Km=0.093mol/L。用浓度质量分数为4%海藻酸钠、体积分数为0.2%戊二醛、0.2mol/L的CaCl2固定多酚氧化酶,固定时间2h,交联时间4h,能使固定化多酚氧化酶的活力达到最大。固定化酶的最适pH为6.0,固定化酶的最佳反应温度为50℃,固定化酶对温度的敏感度降低,温度变化对固定化酶酶活力的影响不大。
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| 关 键 词： | 桑叶 多酚氧化酶 动力学 固定化 |
Study on the immobilization of polyphenol oxidase of mulberry leaf |
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| YANG Yang,HUANG Tao,GUAN Hong-yan,WU Xiao-yu. Study on the immobilization of polyphenol oxidase of mulberry leaf[J]. Science and Technology of Food Industry, 2010, 0(2) |
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| Authors: | YANG Yang HUANG Tao GUAN Hong-yan WU Xiao-yu |
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| Affiliation: | YANG Yang1,HUANG Tao2,GUAN Hong-yan2,WU Xiao-yu1(1. College of Life Science , Technology,Nanning 530004,China,2. College of Light Industry , Food Engineering.,Guangxi University,China) |
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| Abstract: | Kinetic characteristics of polyphenol oxidase(PPO)in mulberry leaf were studied.The result showed that the optimum pH and temperature was 7.0 and 40℃,respectively,and the Michaelisconstant was 0.093mol/L.The immobilization of PP0 from mulberry leaf was researched.The activity of immobilized PPO reached its maximum when 4%sodium alginate,0.2% glutaraldehyde,0.2mol/L CaCl_2 were used to immobilize the PPO,in an immobilization time of 2h and crosslinking time of 4h.The optimum pH and temperature was 6.0,and 50℃ respectively,and the enzyme became less susceptible to the temperature,the temperature did little affect to the enzymatic activity. |
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| Keywords: | mulberry leaf polyphenol oxidase kinetics immobilization |
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