| PDTC对膀胱癌EJ细胞Livin、Survivin mRNA表达影响 |
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| 引用本文： | 张延辉,王充,李树国,刘贤奎,孔垂泽,张哲,陈雪磊,刘校吾,张阁钧.PDTC对膀胱癌EJ细胞Livin、Survivin mRNA表达影响[J].陕西医学杂志,2011,40(5):539-541,546. |
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| 作者姓名： | 张延辉 王充 李树国 刘贤奎 孔垂泽 张哲 陈雪磊 刘校吾 张阁钧 |
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| 作者单位： | 中国医科大学第一附属医院泌尿外科,沈阳,110001 |
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| 基金项目： | 辽宁省科学技术攻关计划 |
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| 摘 要： | 目的:探讨体外细胞培养,观察核转录因子κB通路特异性抑制剂吡咯烷二硫代氨基甲酸酯(PDTC)对膀胱癌EJ细胞凋亡抑制蛋白Livin、Survivin mRNA表达水平的影响。方法:采用Real-time PCR法检测以不同浓度不同时间的PDTC处理的膀胱癌EJ细胞的凋亡抑制蛋白Livin、Survivin mRNA表达水平情况。结果:PDTC可降低膀胱癌EJ细胞凋亡抑制因子Livin、Survivin mRNA表达水平,呈剂量-时间依赖性,且呈负相关(P<0.05)。结论:PDTC可使膀胱癌EJ细胞凋亡抑制蛋白Livin、Survivin mRNA表达水平减少,其机制可能与NF-κB通路被抑制相关。
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| 关 键 词： | 膀胱肿瘤/药物疗法 二硫代氨基甲酸酯/治疗应用 抑制蛋白类/代谢 细胞凋亡 @Livin @Survivin |
Effects of PDTC on the mRNA expression of Livin and Survinin on human bladder carcinoma cell EJ |
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| Affiliation: | Department of Urology,the First Affiliated Hospital of China Medical University(Shenyang 110001)Zhang Yanhui Wang Chong Li Shuguo et al |
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| Abstract: | Objective： Cell culture in vitro,to investigate the effects of PDTC,the selective inhibitor of NF-κB kinase,on mRNA expression of apoptosis inhibited protein Livin and Survivin on human bladder carcinoma cell EJ.Methods： The method of Real-time PCR for detecting the mRNA expressions of Livin and Survivin was used.Results： When PDTC was given,the mRNA expression of Livin and Survivin were down-regulated in a dose-and time-dependent manner（P0.05）.Conclusion： PDTC can down-regulate mRNA expression of apoptosis inhibitory protein Livin and Survivin on bladder carcinoma cell EJ,which might be performed by inhibiting NF-κB signal path by PDTC. |
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| Keywords: | Livin Survivin |
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