| 猪小肠粘膜中肝素钠提取与精制工艺研究 |
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| 引用本文： | 任红媛,何泼,李红心. 猪小肠粘膜中肝素钠提取与精制工艺研究[J]. 食品研究与开发, 2007, 28(1): 78-81 |
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| 作者姓名： | 任红媛 何泼 李红心 |
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| 作者单位： | 陕西科技大学生命科学与工程学院,陕西,咸阳,712081 |
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| 摘 要： | 以猪小肠粘膜为原料，进行肝素钠的提取与纯化，采用两种方法：一种是乙醇沉淀法，另一种是大孔阴离子树脂交换法。主要是通过树脂交换法提取肝素钠粗品，然后采用高锰酸钾和过氧化氢两步氧化法对粗品肝素进行除杂、提纯和精制，最后低温离心得到精品肝素钠。对传统的提取工艺条件进行了改进，并测得粗品平均收率为2．257％，精品平均收率为0．741％，其中粗品效价值为73．19U／mg，精品效价值为146．38U／mg。
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| 关 键 词： | 肝素钠 提取 纯化 |
| 修稿时间： | 2006-04-21 |
STUDY ON TECHNOLOGY OF HERPARIN EXTRACTION AND PURIFICATION FROM SWINE INTESTINAL MUCOSA |
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| REN Hong-yuan,HE Po,LI Hong-xin. STUDY ON TECHNOLOGY OF HERPARIN EXTRACTION AND PURIFICATION FROM SWINE INTESTINAL MUCOSA[J]. Food Research and Developent, 2007, 28(1): 78-81 |
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| Authors: | REN Hong-yuan HE Po LI Hong-xin |
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| Affiliation: | College of Life Science and Engineering, Shaanxi University of Science and Technology, Xianyang 712081, Shaanxi, China |
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| Abstract: | Herparin was extracted and purified from swine intestinal mucosa by two methods, precipitated by alcohol and exchanged by macropore anion-resin. The main experiment task was extracting crude Herparin from swine intestinal mucosa by the second method (resin-exchange) and purifying it by two-step oxidated with potassium permanganate and hydrogen peroxide, then centrifuged at low temperature and gained purified Herparin. Among the article,we got a set of best craft condition and measure the thick Herparin equally accepts the rate as 2.257 %(73.19 U/mg), and the rarefied Herparin equally accepts the rate as 0.741 %(146.38 U/mg). |
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| Keywords: | Herparin extraction purification |
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