玉屏风散总多糖不同给药方式和时相影响小鼠免疫功能的对比研究

目的:比较玉屏风散总多糖(YTP)不同给药方式和时间对免疫低下模型小鼠免疫功能影响的差异。方法:采用环磷酰胺造成免疫低下小鼠模型,YTP用灌胃、静脉注射及静脉注射YTP去蛋白含药血清三种给药方式,观察给药后1、3、14天时对模型小鼠免疫功能的影响。以腹腔巨噬细胞吞噬试验研究药物对非特异性免疫的影响;溶血素抗体法研究受试药物对体液免疫的影响;DNCB所致的迟发型超敏反应研究药物对细胞免疫的影响;免疫荧光染色法测定外周血中CD4 和CD8 T细胞的比例,ELISA测定外周血IL-1、IL-2分泌和呼吸道、肠道灌流液中IgA含量。结果:静脉注射YTP、静脉注射YTP含药去蛋白血清均不能增强腹腔巨噬细胞的吞噬作用和肠道、呼吸道IgA的分泌;灌胃给TYP3天可使肠道IgA分泌增加,但此时呼吸道IgA分泌无明显增加;静脉注射TYP能够在短期内使外周血IL-1、IL-2含量增加,14天时可对特异性免疫产生一定影响。结论:YTP的肠道过程与其对机体非特异性免疫和粘膜免疫功能的增强有关,YTP激活肠道粘膜免疫可能是免疫增强作用的始动环节。

Comparasion studies of Yupingfengsan(玉屏风散) total polysaccharide on immunologic function in mice with different administration methods and periods

Objective: To compare the different effect of Yupingfengsan total polysaccharide(TYP) with different administration methods and periods on immunosuppression mice. Methods: Cyclophosphamide was used to setup the immunosuppression mice model. Compare the immune function on mice model with different administrations of TYP oral administration (ig), intravenous injection (iv) and drug contained serum iv without protein, and different periods of 1,3 and 14 days. Intraperitoneal macrophage phagocytosis experiment was taken to study the drug effect on non-specific immunity; haemolysin antibody experiment was used to study its effect on humoral immunity; DNCB induced delayed hypersensitivity experiment was used to evaluate the cellular immunity effect of the drug; Immunofluorescence was used to determine the scale of CD_4~ and CD_8~ T cell in peripheral blood; and ELISA was used to determine IL-1 and IL-2 secretion in peripheral blood and IgA content in perfusate of the respiratory tract and intestines. Results: TYP iv administration and drug contained serum iv administration without protein did not enhance intraperitoneal macrophage phagocytosis and IgA secretion. TYP ig administration for 3 days increased the IgA secretion in intestinal tract, but not in respiratory tract. TYP iv administration increased the content of IL-1 and IL-2 in peripheral blood in short time, and also affected specific immunity in 14 days. Conclusion: The intestinal process of TYP was related to the enhancement of non-specific immunity and membrane immunity, the immune enhancement of YTP may be caused by the activation on intestinal membrane immunity.