| 电离辐射诱导乳腺癌细胞自噬相关基因DRAM表达的研究 |
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| 引用本文： | 宋志恒,刘晓冬,徐慧英,梁冰,贾立立,孔德娟,易贺庆,贺梦子,马淑梅.电离辐射诱导乳腺癌细胞自噬相关基因DRAM表达的研究[J].中华放射医学与防护杂志,2012,32(1):11-14. |
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| 作者姓名： | 宋志恒 刘晓冬 徐慧英 梁冰 贾立立 孔德娟 易贺庆 贺梦子 马淑梅 |
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| 作者单位： | 吉林大学公共卫生学院,长春,130021 |
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| 基金项目： | 国家自然科学基金(30970682;30770649) |
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| 摘 要： | 目的 研究电离辐射对乳腺癌细胞株MCF-7自噬相关基因DRAM表达变化的影响.方法 采用GFP-LC3转染法检测自噬泡,实时荧光定量PCR方法检测DRAM基因表达,Western blot方法检测LC3和DRAM蛋白的表达,采用磷酸钙共转染法构建DRAM沉默细胞模型.结果 与假照组相比,8 GyX射线照射后细胞中GFP-LC3表达升高；时间-效应关系表明,在8 Gy照射后,自噬溶酶体蛋白LC3表达在2、4、8、16、24和32 h上升变化显著,各组均高于假照组(F=5.38、8.72、10.63、15.23、20.78和55.23,P＜0.05)；DRAM蛋白表达在8 Gy照射后以时间依赖性增加,从2h开始上升,32 h达至最高值(F=116.34,P＜0.05).DRAM沉默细胞模型组中LC3和DRAM蛋白的表达明显低于假照组(F=20.36和40.35,P＜0.05)；对DRAM沉默细胞模型组进行8 Gy照射后,DRAM蛋白表达与假照组相比虽有所增加,但仍低于8 Gy照射组；DRAM沉默细胞模型组LC3蛋白的表达也低于照射组,沉默前后差异均有统计学意义(F=50.34,P＜0.05).结论 X射线可能通过激活DRAM基因来促进乳腺癌细胞株MCF-7发生自噬.
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| 关 键 词： | 自噬 X射线 DRAM基因 |
| 收稿时间： | 2011/4/26 0:00:00 |
Changes of DRAM expression in radiation-induced autophagy in breast cancer cells |
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| SONG Zhi-heng,LIU Xiao-dong,XU Hui-ying,LIANG Bing,JIA Li-li,KONG De-juan,YI He-qing,HE Meng-zi and MA Shu-mei.Changes of DRAM expression in radiation-induced autophagy in breast cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):11-14. |
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| Authors: | SONG Zhi-heng LIU Xiao-dong XU Hui-ying LIANG Bing JIA Li-li KONG De-juan YI He-qing HE Meng-zi and MA Shu-mei |
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| Affiliation: | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China |
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| Abstract: | |
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| Keywords: | Autophagy X-rays DRAM gene |
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