维生素B_6对脑瘫幼鼠认知障碍和步态异常的改善作用

目的:长期维生素B6(VB6)消化道给予脑瘫模型幼鼠,探讨其对认知功能障碍和步态异常的作用及可能机制。方法:14d龄Wistar大鼠54只随机分为脑瘫组(CM,n=18),VB6处理组(CM+VB6,n=18)和假手术组(CS,n=18)。采用改良的Philip方法,建立缺血缺氧脑瘫模型(右侧肢体偏瘫);应用Morris水迷宫试验(MWMT)检测各组大鼠认知功能的变化,观测其寻找水下平台的逃避潜伏期(EL)和游泳距离(SD);应用Footprintpatterntest(FPT)检测各组大鼠步态的变化,观测其左/右侧步长(LSL,RSL)、前肢间距离(FBW)、后肢间距离(HBW)和左/右侧前、后爪心间距离(DLFHFO,DRFHFO)。结果:MWMT:从D12起CM+VB6组EL、SD较CM组缩短(P<0.05)。从D19起CM+VB6组EL与CS组无差异(P>0.05),D20CM+VB6组SD与CS组无差异(P>0.05)。FPT:CM+VB6组与CM组比较,从D5起LSL、RSL增大(P<0.05),从D7起HBW、DRFHFO减小(P<0.05)。CM+VB6组和CS组比较,从D1起RSL、HBWD、RFHFO增大(P<0.05),从D5起LSL减小(P<0.05),D9FBW减小(P<0.05)。结论:VB6对脑瘫幼鼠认知功能障碍、步态异常有改善作用,改善效果随补充时间延长而增强。

THE ROLE OF VITAMIN B6 TO COGNITION AND GAIT DYSFUNCTION IN POSTNATAL RATS WITH CEREBRAL PALSY

Objective:To observe the changes of cognition and gait dysfunction in postnatal rats with cerebral palsy and the possible mechanism.Method:An improved Philips method was used and the model of cerebral palsy was induced.Fifty four Wistar rats aged 14 d with cerebral palsy were randomly divided into 3 groups,cerebral palsy control group(CM,n=18),VB6 treated group(CM VB6,n=18)and sham surgery group(CS).Morris water maze test(MWMT)was used to detect the change of cognition func-tion including the escape latency(EL)and swimming distance(SD).Footprint pattern test(FPT)was used to detect the changes of gaits inctuding the left/right stride length(LSL,RSL),front-base width(FBW),hind-base width(HBW),distance from left/right front footprint/hind footprint overlap(DLFHFO,DRFHFO).Results:MWMT:EL and SD of CM VB6 were shorter than CM's from D 12(P <0.05).EL was indiffer-entiated from D 19,and SD was indifferentiated on D 20 compared with those in CM VB6 and CS group(P >0.05).FPT:LSL and RSL were longer from D 5,and HBW and DRFHFO were shorter from D 7compared with those in CM VB6 and CM group(P <0.05).RSL,HBW and DRFHFO were longer from D 1,and LSL was shorten from D 5,and FBW was shorter on D 9 compared with those in CM VB6 and CS group(P <0.05).Conclusion:VB6 was effective in the improvement of cognition and gait dysfunction in postnatal rats with cerebral palsy,which was much better if treated longer.