| 实时荧光聚合酶链式反应法检测食品中猫源性成分 |
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| 引用本文： | 高晓丽,杨昕霆,王 丹,赵琳娜,陈吉汉,侯彩云.实时荧光聚合酶链式反应法检测食品中猫源性成分[J].食品科学,2014,35(24):235-238. |
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| 作者姓名： | 高晓丽 杨昕霆 王 丹 赵琳娜 陈吉汉 侯彩云 |
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| 作者单位： | 1.中国农业大学食品科学与营养工程学院，教育部-北京市共建功能乳品重点实验室，北京 100083；
2.北京市食品安全监控中心，北京 100041 |
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| 基金项目： | 北京市科技计划课题（Z131100005613005） |
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| 摘 要： | 根据猫线粒体烟酰胺腺嘌呤二核苷酸氧化还原酶亚基1（ND1）基因中的保守序列设计猫特异性引物和TaqMan探针，建立食品中猫源性成分的实时荧光聚合酶链式反应（polymerase chain reaction，PCR）检测方法。通过实时荧光PCR反复验证，结果表明，引物和TaqMan探针对猫源性成分的特异性良好，检测灵敏度可达1 pg，适用于食品中猫源性成分的快速鉴定。通过对随机抽取的市售肉制品的检测，尚未发现掺有猫源性成分的行为。
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| 关 键 词： | 实时荧光聚合酶链式反应 线粒体ND1基因 猫源性成分 |
Detection of Cat-Derived Components in Foods by Real-Time Polymerase Chain Reaction |
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| GAO Xiao-li,YANG Xin-ting,WANG Dan,ZHAO Lin-na,CHEN Ji-han,HOU Cai-yun.Detection of Cat-Derived Components in Foods by Real-Time Polymerase Chain Reaction[J].Food Science,2014,35(24):235-238. |
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| Authors: | GAO Xiao-li YANG Xin-ting WANG Dan ZHAO Lin-na CHEN Ji-han HOU Cai-yun |
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| Affiliation: | 1. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality,
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China |
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| Abstract: | In this study, a TaqMan-based, sensitive and specific real-time polymerase chain reaction (PCR) assay was
developed for the detection of cat-derived components in foods. Primers and TaqMan probe were designed based on the
conserved sequence of the feline mitochondrial ND1, and the performance of the method was invalidated. Results indicated
that no cross-reaction was observed between the feline and non-target species, and this method revealed a high sensitivity and
could detect 1 pg of cat template DNA. In conclusion, the TaqMan probe assay used in this study can be a rapid and sensitive
method for the routine identification of meat species in foods. |
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| Keywords: | real-time PCR mitochondrial ND1 cat-origin component |
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