食品中丙烯酰胺检测新技术研究进展

丙烯酰胺(AA)是一种食品热处理加工过程中产生的具有神经毒性与潜在致癌性的物质,已引起全世界范围的广泛关注。准确测定复杂食品体系中AA的含量,是评估其对人体危害的前提。文章评述了AA传统检测方法(液相色谱,气相色谱,液质联用和气质联用)的不足,主要体现在样品前处理过程复杂、需要必要的衍生化、分析仪器昂贵及需要专业技术人员操作等方面;并重点介绍了毛细管电泳法、酶联免疫吸附试验法、超分子识别法、纳米生物传感法等新型的检测方法的优缺点,及在实际应用中遇到的具体问题;同时,对未来检测方法的发展方向进行展望,旨在为更高效、实用检测方法的开发提供思路。

New determination methods of acrylamide in food products

Acrylamide (AA) is a kind of neurotoxin and potential carcinogen, formed in heating food treatment, has been aroused extensive attention in all over the word. Accurate determination of AA in complex food system is the first importance to evaluate its harmful effects on human health. In this paper, the disadvantages of traditional determination methods of AA, including liquid chromatography, gas chromotography and their coupling technique, were reviewed. Moreover, the complex of sample pretreatment process, requirement of necessary sample derivatives and professional analyzer, and expensive analytical instruments etc. were focused on. Furthermore, several new determination methods, such as capillary electrophoresis, enzyme-linked immunosorbent assay method, super-molecular recognition method, and nano-biosensor, etc. were introduced in detail. The advantages, disadvantages and the existing problems of the current determination methods in practical application were also indicated. Summarizing these methods were helpful to provide thoughts to develop more practical methods. In the future, the promising determination methods should satisfy many merits, including simple sample preparation, rapid and time-saving, low-costing, point-of-care testing and so on.