积雪草甙预防隆乳术后包膜挛缩的细胞及分子机制初探

目的 初步探讨积雪草甙抑制隆乳术后包膜挛缩的细胞及分子机制.方法 将人体来源挛缩包膜组织进行体外分离培养,获得成纤维细胞,将含不同浓度积雪草甙的条件培养基作用于细胞后,采用3H-胸腺嘧啶核苷掺人法、3H-脯氨酸掺入法、Western-blot法分别检测细胞增殖、胶原合成及α-SMA蛋白表达的变化,结果采用SPSS11.0统计学软件分析,并进行t检验.结果 当积雪草甙浓度达到50 mg/L即对细胞DNA合成及胶原合成产生显著的抑制,抑制率分别为34.7%和30.1%,与空白组比较差异具有统计学意义(P＜0.05),随着药物浓度的增加,抑制作用逐渐增强;积雪草甙浓度达到25 mg/L时,α-SMA蛋白表达显著受到抑制,蛋白活化指数为1.673,与空白组比较差异具有统计学意义(P＜0.05).抑制作用与药物浓度正相关.结论 积雪草甙能有效抑制包膜来源成纤维细胞的增殖、胶原合成及向肌成纤维细胞的转分化.

Abstract：

Objective To explore the cellular and molecular mechanism of the inhibitory effect of asciaticoside on capsular contracture following breast augmentation. Methods Contractured capsule derived fibroblasts were cultured in medium with different concentration of asciaticoside. The cell proliferation, collage synthesis and α-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test. Results DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asciaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1%respectively, showing a significant difference from that in control group( P＜0.05 ). The inhibitory effect increased with the rise of the asciaticoside concentration in a dose-dependent manner. When the concentration of asciaticoside reached 25 mg/L, the expression of α-SMA was down-regulated with an activation index of 1. 673, showing a significant difference when compared with that in control group(P＜0.05). Conclusions Asciaticoside can effectively inhibit the DNA and collagen synthesis of capsulederived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.

The cellular and molecular mechanism of inhibitory effect of asciaticoside on capsular contracture following breast augmentation

Objective To explore the cellular and molecular mechanism of the inhibitory effect of asciaticoside on capsular contracture following breast augmentation. Methods Contractured capsule derived fibroblasts were cultured in medium with different concentration of asciaticoside. The cell proliferation, collage synthesis and α-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test. Results DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asciaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1%respectively, showing a significant difference from that in control group( P＜0.05 ). The inhibitory effect increased with the rise of the asciaticoside concentration in a dose-dependent manner. When the concentration of asciaticoside reached 25 mg/L, the expression of α-SMA was down-regulated with an activation index of 1. 673, showing a significant difference when compared with that in control group(P＜0.05). Conclusions Asciaticoside can effectively inhibit the DNA and collagen synthesis of capsulederived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.