超声波辅助提取管花肉苁蓉苯乙醇苷工艺优化及其抗氧化活性研究

为优化超声辅助提取新疆管花肉苁蓉中苯乙醇苷的工艺，在单因素实验的基础上，取提取温度、乙醇浓度、料液比进行响应面优化，得到肉苁蓉苯乙醇苷的最佳提取工艺为:超声温度40 ℃，料液比1:41.7 g/mL、乙醇浓度51%，在该条件下苯乙醇苷得率为(14.36±0.12) mg/g。提取物主要由松果菊苷和毛蕊花糖苷构成，含量分别为(12.96±0.33) mg/g和(1.19±0.09) mg/g。苯乙醇苷提取物对羟基自由基和DPPH自由基具有较强的清除能力，其半抑制浓度（IC₅₀）分别为60.196 mg/mL和0.875 mg/mL。本研究为新疆管花肉苁蓉苯乙醇苷的提取提供了基础数据。

Optimization of Ultrasonic-assisted Extraction of Phenylethanoside from Cistanche tubulosa and Its Antioxidant Activities

In order to optimize the ultrasonic-assisted extraction process of phenylethanol glycosides from Cistanche tubulosa (Ct) in Xinjiang, box-behnken design was used to optimize the extraction conditions based on single factor experiments. Optimal conditions were the temperature of 40 ℃, the solid-liquid ratio of 1:41.7 g/mL and the ethanol concentration of 51%. The extraction yield under the optimal conditions was (14.36±0.12) mg/g. The extracts were mainly composed of echinacoside and verbascoside, the contents of which were (12.96±0.33) mg/g and (1.19±0.09) mg/g, respectively. The phenylethanoside extracts showed strong scavenging ability to hydroxyl radical and DPPH free radical, and the semi-inhibitory concentration (IC₅₀) values were 60.196 mg/mL and 0.875 mg/mL, respectively. This study provides basic data for the extraction of phenylethanoside from Cistanche tubulosa in Xinjiang.