| 基于MBTH法测定溶菌酶对不同脱乙酰度壳聚糖的敏感性 |
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| 引用本文： | 张永勤,常海燕,武 强. 基于MBTH法测定溶菌酶对不同脱乙酰度壳聚糖的敏感性[J]. 化学工业与工程技术, 2014, 0(1): 45-47 |
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| 作者姓名： | 张永勤 常海燕 武 强 |
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| 作者单位： | 青岛科技大学化工学院,山东青岛266042 |
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| 摘 要： | 在优化溶菌酶水解参数的基础上,运用3-甲基-2-苯并噻唑酮腙(MBTH)比色法研究了溶菌酶对不同脱乙酰度的壳聚糖的水解敏感性。结果表明:在70℃、pH值4.5、壳聚糖质量浓度5 mg/mL的条件下,溶菌酶在壳聚糖的脱乙酰度为41.7%时体现出最高敏感性。
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| 关 键 词： | 溶菌酶 壳聚糖 敏感性 MBTH法 |
Study on the susceptibility of lysozyme to the chitosans with different degree of deacetylation based on MBTH assay |
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| ZHANG Yongqin,CHANG Haiyan,WU Qiang. Study on the susceptibility of lysozyme to the chitosans with different degree of deacetylation based on MBTH assay[J]. Journal of Chemical Industry & Engineering, 2014, 0(1): 45-47 |
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| Authors: | ZHANG Yongqin CHANG Haiyan WU Qiang |
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| Affiliation: | (College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China) |
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| Abstract: | Based on optimization of hydrolysis parameters of lysozyme, the susceptibility of lysozyme to the chitosan with different degree of deacetylation is studied by 3-Methyl-2-benzothiazolinonehydrazone （MBTH） assay. The resuhs show that the optimal temperature is 70℃, pH value is 4. 5 and the chitosan concentration is 5 mg/mL. Under the optimum conditions, the susceptibility of lyso- zyme reaches a maximum at 41.7% degree of deacetylation. |
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| Keywords: | lysozyme chitosan susceptibility MBTH assay |
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