水-有机混合介质中电流型生物传感器对葡萄糖的响应

利用自组装单分子层(SAMs)/普鲁士蓝(PB)/壳聚糖(Chit)界面固定葡萄糖氧化酶(GOD),构建酶生物传感器,对传感器在极性非质子有机溶剂如乙腈(AN)、二甲基亚砜(DMSO)和N,N-二甲基甲酰胺(DMF)存在条件下的电化学特性进行了研究,考察了有机溶剂体积分数对传感器响应的影响。结果表明,传感器在50%(体积分数)AN中的电流响应可达到磷酸缓冲溶液(PBS)中的73%。传感器响应速度快、重现性好、抗干扰能力强,展现了高的灵敏度(57.3mA.M.cm-2)。该传感器用于血清标本中葡萄糖的检测,测定结果与市售葡萄糖酶光度测定试剂盒测定结果相吻合。

Response of amperometric biosensor to glucose in aqueous-organic mixed media

A biosensor was fabricated with glucose oxidase （GOD） immobilized on self-assembled monolayers （SAMs）/Prus sian blue （PB）/chitosan （Chit）. The electrochemical characteristics of the biosensor were studied in the presence of dipolar aprotic organic solvents such as acetonitrile （AN）, dimethyl sulfoxide （DMSO） and N, N-dimethyl formamide （DMF）. The effect of volume percentage of organic solvents on biosensor response was investigated. It is observed that the response current of the biosensor in 50% AN reaches 73% of that in phosphate buffer solution （PBS）. The amperometric signals are fast, re producible and exhibiting high sensitivity （57. 3 mA · M · cm-2）. The biosensor was used to determine glucose in serum speci mens, and the results are consistent with those obtained with the commercially available glucose enzyme photometric kit.