| 不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异 |
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| 引用本文： | 田志华,丁慧荣,张宏,张伟,李振甫.不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异[J].北京师范大学学报(自然科学版),2011,47(1):63-68,0,2. |
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| 作者姓名： | 田志华 丁慧荣 张宏 张伟 李振甫 |
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| 作者单位： | 田志华,丁慧荣,张宏,李振甫,TIAN Zhihua,DING Huirong,ZHANG Hong,LI Zhenfu(北京大学临床肿瘤学院,北京肿瘤医院暨北京市肿瘤防治研究所中心实验室,恶性肿瘤发病机制及转化研究教育部重点实验室,100142,北京);张伟,ZHANG Wei(北京师范大学生命科学学院,细胞增殖及调控生物学教育部重点实验室,100875,北京) |
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| 基金项目： | 北京肿瘤医院院内基金资助项目 |
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| 摘 要： | 以人正常胃黏膜上皮细胞系GES-1和不同恶化程度的人胃癌细胞系(低恶化AGS细胞系、中恶化SGC-7901细胞系、高恶化BGC-823细胞系)为研究对象,采用激光共聚焦显微镜的荧光漂白恢复技术及划痕标记荧光染料示踪技术研究不同恶化程度的胃癌细胞系细胞间隙连接通讯(Gap junctional intercellular communication,GJIC)功能的差异;采用荧光定量PCR技术和间接免疫荧光技术检测间隙连接蛋白(Connexin43,Cx43)基因在mRNA和蛋白水平的表达差异,探求其与胃癌恶性程度的相关性.结果表明,在正常胃细胞系和低、中及高恶化胃腺癌细胞系中,细胞间的GJIC功能随着恶性程度的升高而减弱(AGS、SGC-7901)或消失(BGC-823),且Cx43蛋白及mRNA的表达量随着恶性程度的升高而下调(AGS、SGC-7901)或缺失(BGC-823).这提示胃癌恶性程度与胃癌细胞的GJIC功能显著相关(P<0.05),GJIC在胃癌发生中起重要作用.
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| 关 键 词： | 胃癌细胞系 细胞间隙连接通讯(GJIC) 间隙连接蛋白(Cx43) 荧光漂白恢复技术 划痕标记荧光染料示踪技术 荧光定量PCR技术 |
DIFFERENT FUNCTIONS OF GAP JUNCTION INTERCELLULAR COMMUNICATION AND EXPRESSION OF GAP JUNCTION PROTEIN CONNEXIN 43 IN VARIOUS HUMAN GASTRIC CANCER CELL LINES WITH DIFFERENT MALIGNANT DEGREE |
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| TIAN Zhihua DING Huirong ZHANG Hong ZHANG Wei LI ZhenfuKey Laboratory of Carcinogenesis , Translational Research,Ministry of Education,Center Laboratory,Peking University School of Oncology,Beijing Cancer Hospital & Institute,Beijing,China,College of Life Science,Beijing Normal University,Key Laboratory of Cell Proliferation , Regulation Biology of Ministry of Education,China.DIFFERENT FUNCTIONS OF GAP JUNCTION INTERCELLULAR COMMUNICATION AND EXPRESSION OF GAP JUNCTION PROTEIN CONNEXIN 43 IN VARIOUS HUMAN GASTRIC CANCER CELL LINES WITH DIFFERENT MALIGNANT DEGREE[J].Journal of Beijing Normal University(Natural Science),2011,47(1):63-68,0,2. |
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| Authors: | TIAN Zhihua DING Huirong ZHANG Hong ZHANG Wei LI ZhenfuKey Laboratory of Carcinogenesis Translational Research Ministry of Education Center Laboratory Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing China College of Life Science Beijing Normal University Key Laboratory of Cell Proliferation Regulation Biology of Ministry of Education China |
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| Affiliation: | TIAN Zhihua1) DING Huirong1) ZHANG Hong1) ZHANG Wei2) LI Zhenfu1)(1)Key Laboratory of Carcinogenesis and Translational Research,Ministry of Education,Center Laboratory,Peking University School of Oncology,Beijing Cancer Hospital & Institute,100142,Beijing,China,2)College of Life Science,Beijing Normal University,Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education,100875,China) |
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| Abstract: | To correlate function of gap junction intercellular communication(GJIC) and connexin43(Cx43) expression with carcinogenesis,progression and metastasis of gastric cells GES-1(human normal gastric mucosal cell line),AGS(low malignant gastric cell line),SGC-7901(middle malignant gastric cell line),BGC-823(high malignant gastric cell line),GJIC function and Cx43 expression at mRNA and protein levels were investigated using fluorescence recovery after photobleaching(FRAP) under laser scanning confocal microscope... |
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| Keywords: | gastric cancer cell lines GJIC Cx43 fluorescence recovery after photobleaching scrape-loading dye transfer real time quantitative PCR |
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