复方自身清颗粒剂中总芍药苷的定性鉴别以及含量测定

目的对复方自身清颗粒中总芍药苷进行定性鉴别和定量测定。方法定性鉴别采用薄层色谱方法;含量测定采用高效液相色谱法,色谱条件为：色谱柱Agilent C18柱（250 mm×4.6 mm,5μm）,流动相乙腈：0.1%磷酸水（17∶83）,检测波长230 nm,流速1 mL/min,柱温40℃。结果芍药苷在14-140μg/mL范围内与峰面积呈良好的线性关系（r=0.9999）,重现性RSD为2.20%,精密度RSD为0.38%,平均回收率为103.12%（RSD 3.37%）。结论该法简便、准确、可靠,可用于该制剂的质量控制。

The qualitative identification and quantitative detection for total paeoniflorin in Granula Self-cleaning

Objective To establish methods of qualitative identification and quantitative detection for total paeoniflorin in Granula Self-cleaning. Methods TLC and HPLC method were carried out respectively to identify and determine paeoniflorin. The HPLC analysis was performed using SHIM ADZU HPLC system. An Agilent C18 （250 mm × 4.6 mm,5μm） column coupled with guard column C18 （Shim-pack GVP-ODS ×4.6 mm,5 μm） was used at 40 ℃. The mobile phase was consisted of acetonitrile and 0.1% phosphate aqueous （17 ： 83） at flow rate of 1.0 mL · min^-1. The detection wavelength was 230 nm. Results The linear ranges of paeoniflorin was 14 - 140 μg · mL^-1 （r = 0. 999 9） and its average sample recovery of paeoniflorin was 103.12% （ RSD 3.37 % ）. RSD value of reproducibility and precision was respectively 2.20% and 0.38%. Conclusion The established method was simple, accurate, reliable and low cost, thus it is applicable for the quality control of Granula self-cleaning.