连续灌流培养杂交瘤细胞生产单克隆抗体

自 2 0世纪 70年代以来 ,工程抗体在基础医学研究、临床诊断和治疗 ,以及免疫预防等领域中的广泛应用 ,大大促进了其产业化的进程。目前工业化生产单克隆抗体的主要方法是通过发酵罐、中空纤维和固定床等生物反应器培养系统 ,以微载体、微包囊法在体外大规模高密度培养杂交瘤细胞 ,再通过相关的纯化手段浓缩纯化制备抗体[1 ,2 ] 。就操作方式而言 ,一般采用两个基本策略 :①大容量高密度的悬浮培养 ,最多采用的是搅拌式气升式生物反应器 ,通过微载体依托细胞相对固定化 ,降低了搅拌培养时对细胞的剪切力 ,提高细胞的密度和稳定性及生产率。…

Continuous Perfusion Culture Hybridoma Cells for Production of Monoclonal Antibody

Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media. The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amount of production was increased about 2-3 times. The inoculated cell density was 2.5 x 10(5) cells/mL, while the final cell density was 8.79 x 10(8) cells/mL. Antibody production was reached 295 mg/L/d at average level, and the highest level was reached 532 mg/L/d. These results provided a primary mode of enlarge culture for monoclonal antibody industralization.