| 聚丙烯二氧化钛负载膜固定化脂肪酶的研究 |
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| 引用本文： | 汤斌,张庆庆,李松.聚丙烯二氧化钛负载膜固定化脂肪酶的研究[J].食品与发酵工业,2005,31(4):61-63. |
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| 作者姓名： | 汤斌 张庆庆 李松 |
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| 作者单位： | 安徽工程科技学院生化工程系,芜湖,241000 |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 0 2 760 0 1 )，安徽省高校自然科学基金重点资助项目 (No .2 0 0 2kj692d) |
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| 摘 要： | 利用二氧化钛的吸附性,将脂肪酶固定于聚丙烯二氧化钛负载膜上,研究固定化酶的水解特性。结果表明,二氧化钛负载膜上酶的最大负载量为3 0U/cm2 ,固定化酶的最适反应温度为30℃,与自由酶最适温度相比降低了6℃;固定化酶的最适pH为8 7,与原酶最适pH相比,向碱性一侧偏移了0 9个单位;在对大豆油水解时,固定化酶的催化效果比自由酶提高了2 5倍
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| 关 键 词： | 脂肪酶 聚丙烯膜 二氧化钛 固定化 |
| 修稿时间： | 2004年12月10 |
Study of Immobilized Lipase on Polypropylene Membrane with Titanium Dioxide |
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| Tang Bin,Zhang Qingqing,Li Song.Study of Immobilized Lipase on Polypropylene Membrane with Titanium Dioxide[J].Food and Fermentation Industries,2005,31(4):61-63. |
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| Authors: | Tang Bin Zhang Qingqing Li Song |
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| Abstract: | The hydrolytic specificity of the immobilized enzyme has been investigated by immobilizing lipase on polypropylene membrane with titanium dioxide The result showed that the maximal capacity of the membrane for lipase was 3 0U/cm 2 The optimal reaction temperature for the immobilized enzyme was 30℃, which is 6℃ lower than that of using free enzyme alone The optimal pH of the enzyme was 8 7, which drifted 0 9 units to the alkaline side The catalytic effect has been raised 25 fold of that of free enzyme when used to hydrolyzing soybean oils |
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| Keywords: | lipase polypropylene membrane titanium dioxide immobilize |
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