mic2/CD99在经典型霍奇金淋巴瘤H/RS细胞中的表达及与Eber-1/LMP-1相关性的研究

目的： 检测mic2基因与CD99蛋白和Eber-1基因与潜伏膜蛋白-1（LMP1）在经典型霍奇金淋巴瘤（cHL）H/RS细胞中的表达，探讨mic2/CD99表达与Eber-1/LMP-1的相关性。 方法： 采用分子原位杂交和免疫组化技术并结合组织芯片技术检测59例石蜡包埋淋巴瘤组织标本［43例cHL,16例非霍奇金淋巴瘤（NHL）］mic2/CD99和Eber-1/LMP-1的表达，比较分析mic2/CD99在两组中的表达及与Eber-1/LMP-1的关系。 结果： cHL组CD99蛋白表达阳性率为2.3％，mic2基因表达为55.8％，LMP1表达为58.1％，Eber-1表达为53.5％；NHL组CD99蛋白与mic2基因表达高于cHL组（P<0.05）；LMP1和Eber-1的表达低于cHL组（P<0.05）；mic2基因的表达高于CD99蛋白的表达（P<0.05）；Eber-1与LMP1的表达无统计学意义（P>0.05）。CD99蛋白与LMP1的表达呈负相关（P<0.05），各项指标的表达与性别无关。CD99蛋白与LMP1的表达与年龄呈负相关（P<0.05），mic2与Eber-1的表达与年龄无关（P>0.05）。 结论： CD99蛋白在cHL H/RS细胞中低表达，并与LMP1的表达呈负相关。

Expression of mic2/CD99 protein and their correlation with Eber-1/LMP-1 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma

AIM: To study the expression of mic2/CD99 protein and their correlation with Eber-1/LMP-1 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma. METHODS: Immunohistochemical staining, in situ hybrization and tissue microarry technique were used to detect the expressions of mic2/CD99 and Eber-1/LMP-1 of H/RS cells in 43 cases of cHL and 16 cases of NHL. RESULTS: The positive rate of CD99 protein expression in 43 cases of cHL was 2.3％ (1/43), mic2 was 55.8％ (24/43), LMP1 was 58.1％ (25/43) and Eber-1 was 53.5％ (23/43). The expressions of CD99 and mic2 in the NHL group were higher than those in cHL group (P<0.05). The expressions of LMP1 and Eber-1 in NHL group were lower than those in cHL group (P<0.05). The expression of mic2 was higher than CD99 in cHL group (P<0.05). There was no difference between the expression of Eber-1 and LMP1 in cHL group statistically (P>0.05). There was a negative correlation between the expression of CD99 protein and LMP1 in H/RS cells (P<0.05). No correlation was found between the expression of any other markers in H/RS cells and patients' sex (P>0.05). There was a significant correlation between the high expression of LMP1 and a low expression of CD99 in the young patients (P<0.05), but no correlation was found between the expression of mic2 and Eber-1 in young patients (P>0.05). CONCLUSION: There is a negative correlation between the expression of LMP1 and CD99 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma.