茅苍术水溶性成分的HPLC指纹图谱分析

目的:建立茅苍术水溶性成分的HPLC指纹图谱并测定苍术苷A含量,为该药材的质量控制与资源开发提供参考。方法:利用HPLC建立茅苍术的指纹图谱并测定苍术苷A含量,流动相乙腈(A)-0.25%甲酸水溶液(B)梯度洗脱(0~17min,2%~5%A;17~25 min,5%~12%A;25~35 min,12%A;35~50 min,12%~17%A;50~70 min,17%~34%A;70~80min,34%~40%A),检测波长221 nm,流速0.6 m L·min-1。采用外标法测定苍术苷A在10批茅苍术药材中含量。结果:建立了茅苍术水溶性成分的HPLC指纹图谱,检出共有峰18个,精密度、稳定性、重复性试验中各共有峰相对保留时间和相对峰面积的RSD均<3%。10批茅苍术水提物中苍术苷A含量较稳定,平均质量分数2.775 mg·g-1。结论:HPLC指纹图谱方法与苍术苷A的含量测定方法准确、稳定、简便,可有效控制茅苍术药材的内在质量。

HPLC Fingerprint of Water-soluble Components in Atractylodis Rhizoma

Objective: To establish HPLC fingerprints of water-soluble components in Atractylodis Rhizoma and determine the content of atractyloside A. Method: HPLC was adopted to determine the content of atractyloside A with mobile phase of acetonitrile(A)-0.25% aqueous formic acid(B) for gradient elution(0-17 min,2%-5%A;17-25 min,5%-12%A;25-35 min,12%A;35-50 min,12%-17%A;50-70 min,17%-34%A;70-80 min,34%-40%A),detection wavelength at 221 nm and flow rate of 0.6 mL · min^-1.The external standard method was employed to determine the content of atractyloside A in ten batches of Atractylodis Rhizoma. Result: Fingerprint of water extract in Atractylodis Rhizoma was established by HPLC,and 18 common peaks were detected.RSD of common peaks relative retention time and relative peak area in precision,stability and repeatability tests were less than 3%.Water extract of ten batches of samples showed high stability with the content of atractyloside A in Atractylodis Rhizoma was 2.775 mg · g^-1. Conclusion: This established method is specific,accurate and reproducible,it can be used as a quality control method for Atractylodis Rhizoma.