Sequence characterized amplified region markers tightly linked to the dwarf mosaic resistance gene <Emphasis Type="Italic">mdm1 (t)</Emphasis> in maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) |
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Authors: | Cuixia Sun Guangming Zhang Meng Li Xiaopeng Wang Guodong Zhang Yanchen Tian Zeli Wang |
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Affiliation: | (1) National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, 271018, China;(2) College of Plant Protection, Shandong Agricultural University, Tai’an, 271018, China; |
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Abstract: | Maize dwarf mosaic is one of the devastating and wide spread viral diseases in the world. The present investigation was carried
out to develop DNA markers closely linked to the resistance gene mdm1 (t). Linkage between the markers and phenotypes was confirmed by analyzing an F2 population obtained from a cross between a resistant parent ‘Huangzaosi’ and a susceptible parent ‘Mo17(478)’. Four AFLP
markers were found in the maize dwarf mosaic resistant plants. By using (BSA) bulked segregant analysis, two of the four AFLP
markers were transformed into Sequence-characterized amplified regions markers (SCARs), nominated Rsun-1 and Rsun-2. The two
amplified fragment length polymorphism (AFLP) markers, RHC-1and RHC-2, from the amplification products of primer combination
E-AGC/M-CAA and E-AGC/M-GAA, showed linkage with the mdm1 (t) gene in a genetic distance 1.6 and 2.0 cM, respectively. The results indicate that the new SCAR markers will be valuable
to distinguish resistant plants from susceptible plants in plantlets growing in seedbeds. The markers developed in this study
are suitable for marker-assisted selection for maize dwarf mosaic resistance. |
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