铜绿假单胞菌对DBP的降解特性研究

采用小容量全萃取方法，研究了1株铜绿假单胞菌对BDP的降解特性.结果表明，该菌株对DBP具有高效降解能力，当DBP浓度为400 mg/L，投菌量为2 g/L时，t_1/2为3.60　d；其降解过程完全符合一级反应动力学.DBP作为该菌生长的唯一碳源时，其降解过程包括快速生物吸附、解析、降解等几个阶段.实验还确认了存在邻苯二甲酸单丁酯和邻苯二甲酸2种中间产物，从而验证了DBP在双加氧水解酶作用下2步水解变为邻苯二甲酸的历程.

Biodegradation of Di-n-butyl Phthalate by Pseudomonas aeruginosa

A new small capacity-wide extraction method was proposed for detection of its biodegradation in water. Results showed that the half-life (t_1/2) of di-n-butyl phthalate (DBP) biodegradation was 3.60 day when the concentration of DBP was 400 mg/L and the biomass concentration was 2 g/L. The biodegradation process conformed to the first-order kinetic model. Moreover, the whole degradation process could be divided into several steps: adsorption, desorption and degradation. Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry, which confirmed the dioxygenate process during the hydrolysis of DBP.