| 高效液相法测定香菇菇柄麦角甾醇的含量 |
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| 引用本文： | 高虹,程薇,周明,郭鹏,杨德,薛淑静,李露,史德芳. 高效液相法测定香菇菇柄麦角甾醇的含量[J]. 菌物研究, 2013, 11(2): 78-81 |
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| 作者姓名： | 高虹 程薇 周明 郭鹏 杨德 薛淑静 李露 史德芳 |
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| 作者单位： | 湖北省农业科学院农产品加工与核农技术研究所,国家食用菌加工技术研发分中心,湖北省农业科技创新中心农产品加工分中心,武汉430064 |
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| 基金项目： | 公益性行业(农业)专项，湖北省科技研究与开发项目，湖北省公益性科技研究项目 |
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| 摘 要： | 采用高效液相色谱法(High performance liquid chromatography,HPLC)建立了测定菇柄麦角甾醇的含量测定方法。确定提取过程中皂化剂的种类和醇碱比后,将样品皂化,萃取后蒸干溶剂,乙醇定容测定。采用Phe-nomenex-C18色谱柱,V(流动相甲醇)∶V(水)=98∶2,流速1.0 mL/min,检测波长282 nm。结果表明:麦角甾醇线性回归方程为Y=9E+9×106X-8919.9(X:质量浓度,mg/mL),R2=0.998 9,0.01~0.30 mg/mL范围内线性关系良好,回收率为97.31%~101.95%。与紫外分光光度法所测结果比较,HPLC法测定菇柄中麦角甾醇含量灵敏、快速、准确,适用菇柄中麦角甾醇的含量测定。
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| 关 键 词： | 菇柄 麦角甾醇 高效液相色谱法 |
Determination of Ergosterol in Lentinus edodes Stem by High-Performance Liquid Chromatoraphy |
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| GAO Hong,CHENG Wei,ZHOU Ming,GUO Peng,YANG De,XUE Shu-jing,LI Lu,SHI De-fang. Determination of Ergosterol in Lentinus edodes Stem by High-Performance Liquid Chromatoraphy[J]. Journal of Fungal Research, 2013, 11(2): 78-81 |
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| Authors: | GAO Hong CHENG Wei ZHOU Ming GUO Peng YANG De XUE Shu-jing LI Lu SHI De-fang |
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| Affiliation: | (Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology, Hubei A- cademy of Agricultural Sciences, National R ＆ D Center For Edible Fungi Processing, Agricultural Products Processing Subcenter of Hubei Agricultural Science ＆ Technology Innovation Center, Wuhan 430064, China) |
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| Abstract: | A determination method of ergosterol in Lentinus edodes stem by high performance liquid chro- matography （HPLC） was established. After type of saponifier and alcohol-base ratio were determined in the extraction process, the sample was analyzed by saponifiing, extracting to dissolve in ethanol, then de- termined by HPLC. The conditions were Phenomenex-Cls, mobile phase methanol： water = 98：2, 1.0 mL/min velocity, detection wavelength of 282 nm. The calibration curve was Y = 9E ＋ 9 x 106X- 8 919.9 （X：concentration, mg/mL）, R2 = 0.998 9. The standard curve was linear over the range of 0.01-0.3 mg/mL, and the average recovery was 97.31%-101.95%. The results showed that the method is simple and accurate, so it is suitabie for content determination of ergosterol in Lentinus edodes stem. |
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| Keywords: | Lentinus edodes stem ergosterol high performance liquid chromatography |
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