| 绞股蓝对体外培养肾小管细胞庆大霉素毒性的保护作用 |
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| 引用本文： | 黄云剑,廖立生,袁发焕.绞股蓝对体外培养肾小管细胞庆大霉素毒性的保护作用[J].中国中西医结合杂志,1998(Z1). |
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| 作者姓名： | 黄云剑 廖立生 袁发焕 |
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| 作者单位： | 第三军医大学新桥医院肾脏内科 重庆400037 |
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| 摘 要： | 目的:研究绞股蓝(GP)对庆大霉素(GM)引起的近端肾小管细胞(PTC)毒性的保护作用。方法:采用原代培养的 PTC 制作 GM 损伤模型,观察丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、N-乙酰-β-氨基葡萄糖酶(NAG)、过氧化氢酶(catalase)、DNA 合成以及形态学变化,同时观察 GP 的保护作用。结果:GP 能激活并保护 ALT、LDH、Catalase 酶活力,降低 NAG酶活力增高、促进 DNA 合成等机制,减轻 GM 的肾毒性损伤。结论:GP 能有效保护 GM 对PTC 损伤。
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| 关 键 词： | 绞股蓝 庆大霉素 近端肾小管细胞 |
The Protective Effect of Gypenoside on Gentamicin-Induced Renal Proximal Tubular Cell Toxicity in vitro |
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| Authors: | Huang Yunjian Liao Lisheng Yuan Fahuan Deptof Nephrology Xinqiao Hospital The Third Military Medical University Chongqing |
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| Abstract: | Objective:To investigate the protective effect of gypenoside(GP)on gentamicin(GM)-induced nephrotoxicity. Methods:Alanine aminopeptidase(ALT),LDH,catalase,and N-acetyl-(-glucosaminidase(NAG)activities,DNA syn- thesis and morphological alterations were observed in GM-induced damage to cultured primary renal tubular cells(PTC). The protection of GP to injury was observed,too.Results:GP notably ameliorated GM-induced damage through the mechanism of restoring ALT,DH,catalase activities,reducing NAG activity,promoting DNA synthesis.Conclusion: GP could efficiently protect renal proximal tubular cells from GM nephrotoxicity. |
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| Keywords: | gypenoside gentamicin renal proximal tubular cell |
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