垂枝樱花外植体灭菌及不定芽的诱导研究 |
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引用本文: | 王红梅,李园莉,李洪光,董晓辉. 垂枝樱花外植体灭菌及不定芽的诱导研究[J]. 安徽农业科学, 2014, 0(35): 12421-12422,12439 |
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作者姓名: | 王红梅 李园莉 李洪光 董晓辉 |
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作者单位: | 江苏农林职业技术学院,江苏句容,212400;嘉汉城市生态苗木(苏州)有限公司,江苏苏州,215028 |
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基金项目: | 江苏农林职业技术学院科技项目“垂枝樱花栽培繁殖技术体系研究” |
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摘 要: | [目的]探讨垂枝樱花外植体灭菌及不定芽诱导的相关技术.[方法]以垂枝樱花当年生带腋芽茎段为外植体,对其组织培养繁殖技术中外植体选择、灭菌方法与程序、不定芽的诱导等环节进行了初步研究.[结果]适宜外植体采集时间为晴天中午,采集部位为当年生枝条的中上部,适宜的表面灭菌程序为:洗衣粉中浸泡15 min,流水冲洗lh,70%乙醇浸泡30 s,0.1% HgCl2浸泡10 min,最后无菌水冲洗5遍,成活率达68.3%.适宜垂枝樱花不定芽诱导的培养基为1/2MS +6-BA 0.5 mg/L+ NAA 0.10 mg/L,分化率达68.3%.[结论]为垂枝樱花的组织培养提供相关技术支持.
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关 键 词: | 垂枝樱花 组织培养 培养基 表面灭菌 |
The Study on Sterilization of Explant and Formation of Adventitious Bud for Cerasus subhirtella Miq.var.pendula Tanaka. |
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Affiliation: | WANG Hong-mei;LI Yuan-li;LI Hong-guang;Jiangsu Polytechnic College of Agriculture and Forestry;Jiahan Urban Ecology Seedings(Soochow) Co. Ltd; |
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Abstract: | [Objective] The aim was to preliminaryly discuss sterilization of explant and formation of adventitious bud for Cerasus subhirtella Miq.var.pendula Tanaka.[Method] With the stems with buds of Cerasus subhirtella Miq.var.pendula Tanaka.as explants,the explant selection,methods and procedures of sterilization and adventitious bud induction were studied.[Result] The results showed that,the suitable explants acquisition time of midday,collectionsite was in the upper part of annual shoots; the procedures of surface sterilization was immersing 15min of washing powder,water flushing 1 h,70% ethand soaking 30 s,0.1% HgC12 immerising 10 min,the sterile water rinsing 5 times,and reached the survival rate of 66%.The suitable inducing medium was 1/2MS + 6-BA 0.5 mg/L + NAA 0.10 mg/L,and the differentiation rate reached 63.6%.[Conclusion] The study provided related technical support for tissue culture of Cerasus subhirtella Miq.var.pendula Tanaka. |
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Keywords: | Cerasus subhirtella Miq.var.pendula Tanaka. Tissue culture Culture medium Surface sterilization |
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