| 藤梨根甲醇提取物的抗氧化活性及物质基础初步研究 |
| |
| 引用本文： | 滕坤,张海丰,臧皓,沈鹏,孙佳明.藤梨根甲醇提取物的抗氧化活性及物质基础初步研究[J].中草药,2019,50(18):4384-4388. |
| |
| 作者姓名： | 滕坤 张海丰 臧皓 沈鹏 孙佳明 |
| |
| 作者单位： | 宁波卫生职业技术学院医学技术学院, 浙江 宁波 315100,宁波卫生职业技术学院医学技术学院, 浙江 宁波 315100,通化师范学院医药学院, 吉林 通化 134002,通化师范学院医药学院, 吉林 通化 134002,长春中医药大学吉林省人参科学研究院, 吉林 长春 130117 |
| |
| 基金项目： | 国家自然科学基金资助项目（31100254）；吉林省教育厅项目：基于细胞药代动力学的藤梨根抗肿瘤黄酮类成分研究（吉教科合字[2015]第439号） |
| |
| 摘 要： | 目的研究藤梨根甲醇提取物的抗氧化活性,并初步分析其活性化学成分。方法以清除1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基能力为指标,测定藤梨根甲醇提取物的体外抗氧化活性;采用高效液相色谱-质谱联用技术对藤梨根甲醇提取物化学成分进行分析。结果藤梨根甲醇提取物对DPPH、ABTS自由基具有良好的清除作用,半数有效浓度(EC50)分别为(26.275±1.464)mg/mL和(29.826±1.309)mg/mL;通过紫外光谱和质谱分析,初步鉴定了19个化学成分。结论藤梨根甲醇提取物具有很好的体外抗氧化活性,其主要活性成分为多羟基和不饱和双键的成分。
|
| 关 键 词： | 藤梨根 抗氧化 HPLC-DAD-ESI-MS/MS 1, 1-二苯基-2-三硝基苯肼 2, 2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)二铵盐 |
| 收稿时间： | 2019/1/4 0:00:00 |
Antioxidant activities in vitro and components analysis of methanol extract of Actinidia arguta by HPLC-DAD-ESI-MS/MS |
| |
| TENG Kun,ZHANG Hai-feng,ZANG Hao,SHEN Peng and SUN Jia-ming.Antioxidant activities in vitro and components analysis of methanol extract of Actinidia arguta by HPLC-DAD-ESI-MS/MS[J].Chinese Traditional and Herbal Drugs,2019,50(18):4384-4388. |
| |
| Authors: | TENG Kun ZHANG Hai-feng ZANG Hao SHEN Peng and SUN Jia-ming |
| |
| Affiliation: | College of Medical Technology, Ningbo College of Health Sciences, Ningbo 315100, China,College of Medical Technology, Ningbo College of Health Sciences, Ningbo 315100, China,College of Pharmaceutical, Tonghua Normal University, Tonghua 134002, China,College of Pharmaceutical, Tonghua Normal University, Tonghua 134002, China and Jilin Ginseng Academy, Changchun University of Traditional Chinese Medicine, Changchun 130117, China |
| |
| Abstract: | Objective To investigate and analyze antioxidant activities in vitro and the active ingredients of the methanol extract of Actinidia arguta. Methods In this study, antioxidant activities of extract of A. arguta were carried out by using DPPH and ABTS radical scavenging assays in vitro. Qualitative analysis of major active components was performed by HPLC-DAD-ESI-MS/MS. Results Extract of A. arguta had good scavenging effect on DPPH and ABTS free radical in vitro, and the EC50 values of scavenging effect of extract of A. arguta on DPPH and ABTS free radical were (26.275 ±1.464) and (29.826 ±1.309) mg/mL, respectively. On the basis of UV and mass spectral analysis, a total of 19 chemical compositions were preliminarily identified. Conclusion extract of A. arguta has good antioxidant activity in vitro, and polyhydroxyl and unsaturated double bonds are the main active constituents. |
| |
| Keywords: | Actinidia arguta (Sieb et Zucc ) Planch ex Miq antioxidant HPLC-DAD-ESI-MS/MS DPPH ABTS |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
