| 一种新型T细胞免疫原的原核表达及对口蹄疫疫苗的免疫增强作用 |
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| 引用本文： | 赵清,孙普,刘在新,李平花,包慧芳,曹轶梅,白兴文,付元芳,卢曾军,李冬.一种新型T细胞免疫原的原核表达及对口蹄疫疫苗的免疫增强作用[J].生物工程学报,2011,27(9):1281-1291. |
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| 作者姓名： | 赵清 孙普 刘在新 李平花 包慧芳 曹轶梅 白兴文 付元芳 卢曾军 李冬 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,兰州,730046 |
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| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2006AA10A204)，甘肃省农业生物技术与应用开发项目 (No. GNSW-2009-10) 资助。 |
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| 摘 要： | 主要探讨了T细胞免疫原TI对口蹄疫疫苗的免疫增强作用。设计并原核表达产生了一种包含口蹄疫病毒VP1，VP4，3A和3D蛋白上多个T细胞表位与两个通用T细胞表位的T细胞免疫原，命名为TI；同时表达了O和Asia 1两个型口蹄疫病毒 VP1 蛋白的串联编码基因，表达产物命名为OA-VP1。将上述T细胞免疫原分别与OA-VP1和口蹄疫灭活疫苗按不同剂量组合免疫小鼠，于免疫后不同时间测定各组小鼠的体液与细胞免疫应答情况。采用微量中和试验检测小鼠O型和Asia1型中和抗体，采用流式细胞检测技术和测定γ-干扰素的水平来分析不同免疫组小鼠细胞免疫的水平。结果显示，与灭活疫苗或OA-VP1单独免疫组相比，添加TI抗原后灭活疫苗 (P＜0.01) 和OA-VP1免疫组(P＜0.05)小鼠均能产生高水平的特异性中和抗体；且CD4+ T细胞数量显著增多，IFN-γ产生水平显著升高 (P＜0.01)。说明TI抗原具有很好的诱导特异性体液与细胞免疫应答的作用，是一种很好的免疫增效剂，可作为口蹄疫蛋白亚单位疫苗和灭活疫苗中的一种有效成分，以提高疫苗的免疫效果。
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| 关 键 词： | 口蹄疫病毒，T细胞表位，T细胞免疫原，灭活疫苗，亚单位疫苗 |
| 收稿时间： | 2011/1/31 0:00:00 |
Enhanced immune response of a novel T-cell immunogen in vaccinefor foot-and-mouth disease |
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| Qing Zhao,Pu Sun,Pu Sun,Pinghua Li,Huifang Bao,Yimei Cao,Xingwen Bai,Yuanfang Fu,Zengjun Lu and Dong Li.Enhanced immune response of a novel T-cell immunogen in vaccinefor foot-and-mouth disease[J].Chinese Journal of Biotechnology,2011,27(9):1281-1291. |
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| Authors: | Qing Zhao Pu Sun Pu Sun Pinghua Li Huifang Bao Yimei Cao Xingwen Bai Yuanfang Fu Zengjun Lu and Dong Li |
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| Affiliation: | National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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| Abstract: | We investigated the enhanced immune response of a recombinant T cell immunogen as an effective cellular immune adjuvant.The T cell immunogen named TI contained several T cell epitopes from the VP1,VP4,3A and 3D proteins of foot-and-mouth disease virus(FMDV) and two pan-T helper(TH) cell sites to broaden the immunogenicity of the protein.Meanwhile,another fusion protein named OA-VP1 was expressed in bacteria,which contained two VP1 proteins of O and Asia1 type FMDV.Mice were vaccinated with commercially inac... |
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| Keywords: | foot-and-mouth disease virus T cell epitopes T cell immunogen inactivated vaccine subunit vaccine |
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