HSP70-1、HSP70-2基因多态性与肺癌易感性的相关性

目的]探讨热休克蛋白HSP70-1＋190（G／C）、HSP70．2＋1267（A／G）两位点基因多态性与肺癌易感性的相关性。方法]采用病例．对照研究，以病理确诊的肺癌患者159例为病例组，202名正常体检人群为对照组。应用聚合酶链反应．限制性片段长度多态性分析（PCR—RFLP）技术检测HSP70-1＋190（G／C）和HSP70-2＋1267（A／G）的单核苷酸多态性。采用非条件Logistic回归分析两位点基因型与吸烟的交互作用及两位点之间的交互作用。结果]HSP70-1＋190（G／C）位点的3种基因型GG、GC和CC及G和C等位基因频率在病例组和对照组之间的分布，差异均有统计学意义（χ^2=19．16，P〈0．05；χ^2=18．76，P〈0．05），CC基因型与肺癌具有中等强度关联。HSP70-2＋1267（A／G）位点的基因型及等位基因的分布在两组之间差异均无统计学意义（P〉0．05）。按照吸烟情况进行分层分析发现，重度吸烟人群中携带HSP70-1＋190（G／C）位点的GC基因型和GC＋CC基因型者及HSP70—2＋1267（A／G）位点的删基因型的个体患肺癌的风险增高。基因型与吸烟的交互作用分析发现，HSP70-1＋190（G／C）位点多态性和吸烟具有相乘交互作用（P=0．01）。HSP70-1＋190（G／C）和HSP70．2＋1267（A／G）两位点多态性之间存在交互作用（χ^2=12．87，P=0．00）。结论]HSP70-1＋190（G／C）位点多态性可能与河南汉族人群中肺癌易感性有关，与HSP70-2＋1267（A／G）和吸烟均具有相乘交互作用；HSP70-2＋1267（A／G）位点多态性与肺癌的相关性还有待进一步研究，其州基因型可能与重度吸烟之间存在交互作用。

The Heat Shock Protein HSP70-1 and HSP70-2 Gene Polymorphism and Susceptibility to Lung Cancer

Objective ] To investigate the association between the heat shock protein 70 gene polymorphism and lung cancer. Methods ] A case-control study was conducted among 159 lung cancer patients and 202 control subjects. Polymerase chain reaction-restrained fragment length polymorphism technique （ PCR-RFLP ） was applied to detect the single nucleotide polymorphisms （ SNPs ） of HSP70-1 ＋190 （ G/C ） and HSP70-2 ＋1267 （ A/G ）. The interactions of the two gene polymorphisms and smoking and that of the two sites were analyzed by non-conditional Logistic regression. Results ] There were significant differences between the case group and control group（χ^2= 19.16, P 〈 0.05;χ^2 = 18.76, P 〈 0.05 ）in the distribution of each genotypes GG, GC and CC, and also in the frequencies of the two alleles G and C of HSPTO-1 ＋190（ G/C ）. The CC genotype was moderately correlated with lung cancer. There were no significant differences in the distribution of genotypes and allele frequencies of HSP70-2 ＋ 1267 （ A/G ） between the case group and control group （ P 〉 0.05 ）. After stratification analysis according to smoking, it revealed that in heavysmokers subgroups who carried HSP70-1 ＋190（ G/C ）GC or GC＋CC genotypes and/or HSP70-2 ＋1267（ A/G ）AA genotype were associated with significantly increased risk of lung cancer. The interaction analysis of genotype and smoking revealed that there was multiplication interaction between HSP70-1 ＋190 （ G/C ）polymorphism and smoking on susceptibility to lung cancer. There was also interaction between the two genotypes HSP70-1 ＋190（ G/C ）and HSP70-2 ＋1267（ A/G χ^2 = 12.87, P = 0.00 ）. Conclusion ] HSP70-] ＋190（ G/C ）polymorphism may be associated with the susceptibility to lung cancer in He＇nan Han population, and there are multiplication interaction with HSP70-2 ＋1267 （A/G）polymorphism and smoking. The relationship between HSP70-2 ＋1267 （A/G） polymorphism and lung cancer has yet to be further studied and there may be an interaction between AA genotype and the heavy smokers.