海洋扩展青霉(Penicillium expansum)产耐盐型果胶酶的固态发酵优化

目的:提高海洋扩展青霉(Penicillium expansum)产耐盐型果胶酶的酶活力。方法:测定海洋扩展青霉所产果胶酶的耐盐性;通过单因素和正交实验,对海洋扩展青霉产耐盐型果胶酶进行固态发酵优化;利用SPSS 20.0分析实验结果。结果:该果胶酶在底物所含Na Cl浓度为30g/L时,酶活力最高,在Na Cl浓度为0~60g/L范围内较稳定。最优培养基为:麸皮7g,果胶粉0.42g,氯化铵0.28g,人工海水8.4m L;最优培养条件为:接种量3m L(107cfu/m L),培养温度28℃,250m L三角瓶中培养72h。此优化条件下酶活力可达到6639.79U/g。结论:该果胶酶耐盐性好;固态发酵优化后,酶活力达到6639.79U/g,为原来的酶活1239.80U/g的5.36倍。 

Optimization of solid fermentation conditions of marine Penicillium expansum for producing salt tolerant pectinase

Objective:To improve the capacity of marine Penicillium expansum for producing salt tolerant pectinase.Methods:Salt tolerance of pectinase from marine Penicillium expansum was investigated. Solid fermentation conditions of pectinase from marine Penicillium expansum were optimized by single factor and orthogonal experiments. Statistical data was analyzed by SPSS 20.0. Results :Pectinase activity was the highest at the concentration of 30g/L Na Cl,and was stable in 0~60g/L Na Cl. The optimal condition for Penicillium expansum was composed of bran 7g,jelly powders 0.42 g,ammonium chloride 0.28 g and artificial seawater 8.4m L,and was cultured at 28℃ for 72 h with 3m L(107cfu/m L) inoculation in each 250 m L triangle flask. The pectinase activity could reach 6643.49U/g. Conclusion :The pectinase had good salt tolerance. Pectinase activity could reach6643.49U/g under optimized conditions,which was 5.36 times more than that at the initial conditions with1239.80U/g.