一氧化碳释放分子3对缺氧/复氧心肌细胞内活性氧的影响

目的探讨一氧化碳释放分子3(CORM-3)对缺氧/复氧心肌细胞内活性氧(ROS)产生的影响。 方法对大鼠H9c2心肌细胞缺氧6 h后，分别以10 μmol/L、50 μmol/L、100 μmol/L、200 μmol/L的CORM-3复氧6 h，检测心肌细胞内ROS量，观察浓度效应。以50 μmol/L CORM-3对缺氧6 h后的H9c2心肌细胞分别进行2 h、4 h、6 h、10 h复氧处理，检测心肌细胞内ROS量，观察时间效应。 结果当CORM-3浓度为50 μmol/L、100 μmol/L时，心肌细胞内ROS量显著降低，其中50 μmol/L的CORM-3效果更好，即ROS量下降最明显；当CORM-3浓度为200 μmol/L时，心肌细胞内ROS量反而升高。50 μmol/L CORM-3在复氧6 h、10 h都能显著降低H9c2心肌细胞的ROS量，其中复氧6 h的效果更好，即ROS量下降最明显。 结论适当浓度的CORM-3能显著降低缺氧/复氧心肌细胞内ROS量。CORM-3降低缺氧/复氧心肌细胞内ROS量与复氧时间相关。

Effects of water-soluble CO-releasing molecule on hypoxia/reoxygenation in myocardial cells

ObjectiveTo observe the effects of water-soluble CO-releasing molecules(CORM-3) on reactive oxygen species(ROS)in myocardial cells after hypoxia/reoxygenation. MethodsAfter hypoxia for 6h, H9c2 cellswere treated with CORM-3 in 10 μmol/L, 50 μmol/L, 100 μmol/L and 200 μmol/L, respectively, and then the effect was evaluated by detecting the changesof ROS. The content of ROS was determined in H9c2 cell after 2 h, 4 h, 6 h and 10 h for reoxygenation with 50 μmol/L of CORM-3 to evaluate its effect of reoxygenation time. ResultsThe content of ROS in 50 μmol/L and 100 μmol/L groupswassignificantly decreased, and the effect was better in 50 μmol/L group. The contentof ROS was significantly decreased in 6h and 10 h reoxygenation groups, and the effect was more significant in 6 h reoxygenation group. ConclusionThe appropriate concentration of CORM-3 can decrease ROS in hypoxia/reoxygenation of myocardial cells. The effect of CORM-3 in decreasing ROS is correlated with the reoxygenation time.