| 脱氧核糖核酸分子设计在电化学生物传感器中的应用 |
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| 引用本文： | 刘刚,万莹,邹子英,任淑贞,樊春海. 脱氧核糖核酸分子设计在电化学生物传感器中的应用[J]. 分析化学, 2011, 39(7): 953-962. DOI: 10.1016/S1872-2040(10)60449-5 |
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| 作者姓名： | 刘刚 万莹 邹子英 任淑贞 樊春海 |
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| 作者单位： | 上海市计量测试技术研究院;中国科学院上海应用物理研究所物理生物学实验室; |
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| 基金项目： | 国家自然科学基金(No.20725516); 国家质检总局科技计划项目(No.HOORJ906); 上海市科委科研计划项目(No.10142201700)资助 |
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| 摘 要： | 基于特殊DNA序列的构型变化的电化学生物传感器是一种高灵敏、高特异性的生物分析方法.固定在电极表面的特殊DNA探针(茎环、核酸适配体、四聚体等)因为目标物质的结合而发生构型变化,从而产生可检测的电化学信号,这种策略操作简便而且特异性强,引起了研究者的广泛关注.本文总结了目前基于基因构型变化的电化学生物传感器的发展历程.
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| 关 键 词： | 电化学传感器 基因传感器 核酸适配体 分子信标 评述 |
| 收稿时间： | 2010-10-18 |
Deoxyribonucleic Acid Molecular Design for Electrochemical Biosensors |
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| LIU Gang,WAN Ying,ZOU Zi-Ying,REN Shu-Zhen,FAN Chun-Hai. Deoxyribonucleic Acid Molecular Design for Electrochemical Biosensors[J]. Chinese Journal of Analytical Chemistry, 2011, 39(7): 953-962. DOI: 10.1016/S1872-2040(10)60449-5 |
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| Authors: | LIU Gang WAN Ying ZOU Zi-Ying REN Shu-Zhen FAN Chun-Hai |
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| Affiliation: | aState Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China;bLaboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China |
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| Abstract: | This review presents a novel kind of electrochemical biosensors based on DNA configuration switch. Special DNA probes (stem-loop or aptamers etc.) fixed on an electrode surface dramatically change their configuration on the combination of the target molecule giving birth to a detectable electrochemical signal. This strategy attracts enormous research interest because of its easier operation and higher specificity. To this day, its analysis performance has been markedly developed with an expanding application. We here summarize the development of these sensors. |
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| Keywords: | Elechemical biosensor DNA biosensor Aptamer Molecular beacon Review |
| 本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录！ |
