3种三七样品中皂苷类成分的大鼠在体肠吸收特性比较

目的:比较三七原枝粉、超微粉、破壁粉中三七皂苷R1,人参皂苷Rg_1,人参皂苷Rb_1的大鼠在体小肠吸收差异,为该药材的原料筛选提供依据。方法:采用大鼠在体循环灌流法,运用HPLC同时测定肠循环灌流液中三七皂苷R1,人参皂苷Rg_1,人参皂苷Rb_1及酚红的含量,研究3种三七样品中三七皂苷R1,人参皂苷Rg_1和人参皂苷Rb_1在大鼠小肠的吸收情况。结果:三七原枝粉、超微粉、破壁粉中三七皂苷R1的吸收速率常数(K_a)分别为0.049 80,0.037 37,0.034 60 h~(-1),人参皂苷Rg_1的K_a分别为0.044 83,0.032 48,0.036 50 h~(-1),人参皂苷Rb_1的K_a分别为0.078 40,0.095 48,0.084 78 h~(-1);三七皂苷R1的吸收率(P)分别为9.543%,7.662%,5.858%,人参皂苷Rg_1的P分别为8.602%,7.154%,6.667%,人参皂苷Rb_1的P分别为14.60%,17.86%,15.64%。对于三七皂苷R1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变小且存在显著性差异;对于人参皂苷Rg_1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变小;对于人参皂苷Rb_1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变大且超微粉存在显著性差异。结论:3种三七粉中三七皂苷R1,人参皂苷Rg_1和人参皂苷Rb_1在大鼠小肠的吸收量和吸收速率均存在一定差异,但三七超微粉和破壁粉的吸收情况并不比三七原枝粉好,建议原料选择三七原枝粉。

Comparison of in Situ Intestinal Absorption in Rats of Saponins in Three Kinds of Notoginseng Radix et Rhizoma Samples

Objective: To compare the difference of in situ small intestinal absorption in rats of notoginsenoside R₁,ginsenoside Rg₁ and ginsenoside Rb₁ in original powder, ultra-fine powder and cell wall-broken powder of Notoginseng Radix et Rhizoma. Method: In situ perfusion method in rats was adopted,the concentration of notoginsenoside R₁,ginsenoside Rg₁,ginsenoside Rb₁ and phenol sulfonphthalein in intestinal circulating perfusate were determined simultaneously by HPLC,which is used to study on the absorption of notoginsenoside R₁,ginsenoside Rg₁and ginsenoside Rb₁ from Notoginseng Radix et Rhizoma samples in the small intestine of rats. Result: The absorption rate constants (K_a) of notoginsenoside R₁ in the original powder,ultra-fine powder and cell wall-broken powder were 0.049 80,0.037 37,0.034 60 h^-1,K_a of ginsenoside Rg₁ were 0.044 83,0.032 48,0.036 50 h^-1,K_a of ginsenoside Rb₁ were 0.078 40,0.095 48,0.084 78 h^-1,respectively.The absorption percentages (P) of notoginsenoside R₁ were 9.543%,7.662%,5.858%,those of ginsenoside Rg₁ were 8.602%,7.154%,6.667%,those of ginsenoside Rb₁ were 14.60%,17.86% and 15.64%,respectively. Conclusion: There are differences in the absorption amount and rate of notoginsenoside R₁,ginsenoside Rg₁ and ginsenoside Rb₁ in three kinds of Notoginseng Radix et Rhizoma powder,but the absorption of ultra-fine powder and cell wall-broken powder has no significant superiority by comparing with the original powder,selection of original powder as raw material is better.