G-四联体结构在生物传感器中的应用进展

G-四联体结构是由多聚鸟嘌呤序列通过Hoogsteen氢键配对形成的笼式结构.该结构具有独特的化学性质,如可以特异性地与N-甲基卟啉二丙酸Ⅸ(NMM)化合物结合,产生荧光以及具有过氧化物酶活性等.据此科学家开发了一些生物传感器,并取得了丰硕的成果.此类传感器大多是通过被检物质引起DNA结构的变化,进而引起荧光信号和过氧化物酶活性变化,最终达到检测的目的.对基于G-四联体结构开发的生物传感器检测金属离子、有机大分子、核酸和生物酶等物质的研究进展进行了综述.

Progress of G-Quadruplexes Application in Biosensors

G-Quadruplexes are formed by the stacking of guanine-rich sequences of DNA composed of four guanines paired by Hoogsteen hydrogen bonding.The structure has the unique chemical properties,such as producing fluorescence by binding with Nmethyl mesoporphyrin Ⅸ (NMM) and peroxidase activity.These properties are used to develop biosensors and a lot of biosensors are developed.Principles of G-quadruplex-based detection methods involve DNA conformational change caused by the presence of analyte which leads to a decrease or an increase in fluorescence and peroxidase activity of the used probe.It is a potential method in analysis and detection.This work mainly reviewed the technology and advances of G-quadruplexes used in development of biosensors to detect metal ion,organic macromolecule,nucleic acid and biological enzyme.