| 从小麦胚芽蛋白中分离和鉴定血管紧张素转化酶抑制肽的研究 |
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| 引用本文： | 辛志宏,马海乐,吴守一,骆琳. 从小麦胚芽蛋白中分离和鉴定血管紧张素转化酶抑制肽的研究[J]. 食品科学, 2003, 24(7): 130-133 |
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| 作者姓名： | 辛志宏 马海乐 吴守一 骆琳 |
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| 作者单位： | 江苏大学生物与环境工程学院 |
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| 摘 要： | 用碱性蛋白酶水解小麦胚芽蛋白得到的水解物对血管紧张素转化酶有强的抑制活性(IC50=0.014mg/ml),小麦胚芽蛋白水解物经SephadexG-15纯化、制备RP-HPLC分离,得一对ACE有强烈抑制作用的组分X(IC50=5.46μmol/L),X氨基酸分析、电喷雾质谱确定了X的序列为Ala-Met-Tyr。
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| 关 键 词： | 小麦胚芽蛋白 血管紧张素转化酶抑制肽 电喷雾质谱 |
| 文章编号： | 1002-6630(2003)07-0130-04 |
Study on Extraction and Assay of the Angiotension-converting Enzyme Inhibitory Activity from Wheat Germ Embryo Protein |
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| XIN Zhi-Hong,MA Hai-Le,WU Shou-Yi,LUO Lin. Study on Extraction and Assay of the Angiotension-converting Enzyme Inhibitory Activity from Wheat Germ Embryo Protein[J]. Food Science, 2003, 24(7): 130-133 |
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| Authors: | XIN Zhi-Hong MA Hai-Le WU Shou-Yi LUO Lin |
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| Abstract: | Aldaline protease hydrolyzate extracted from wheat germ embryo protein showed a high angiotensin I-convertingenzyme inhibitory activity (IC50=0.014mg/ml).A high ACE inhibitory fraction X (IC50=5.46μmol/L) was obtained by theconsecutive separation methods including Sephadex G-15 chromatography and preparation reverse-phase high performance liquidchromatography.The sequence of X was found as Ala-Met-Tyr after the application of amine acid composition assay andelectrospray mass spectrometry. |
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| Keywords: | wheat germ embryo protein angiotensin I-converting enzyme inhibitory peptide electrospray mass spectrometry |
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