wt-P53蛋白对人瘢痕疙瘩成纤维细胞端粒酶活性的影响

目的 探讨wt-P53蛋白对人瘢痕疙瘩成纤维细胞（keloidfibroblasts，KFBS）端粒酶活性的影响；明确在人KFBs中wt-P53蛋白与端粒酶活性之间的相互关系。方法 将来源于人瘢痕疙瘩组织的KFBs随机分成两组，转染组采用腺病毒介导法将野生型Wt-p53基因转染至人KFBs；非转染组KFBs未进行野生型wt-p53基因转染。转染48h后，采用间接免疫荧光法和Western blotting法检测KFBs wt-P53蛋白的表达；并于转染后1～7d，采用TRAP—ELISA法检测KFBs端粒酶活性。结果两组均有wt-P53蛋白表达，转染组Wt-P53蛋白表达明显高于非转染组；转染后1～7d，转染组端粒酶活性均明显低于非转染组（P〈O．05）。结论 wt-P53蛋白能够抑制人KFBs端粒酶活性。

EFFECT OF wt-P53 PROTEIN ON TELOMERASE ACTIVITY IN KELOID FIBROBLASTS

Objective To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts(KFBs).Methods The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis.KFBs were divided into 2 groups,the transfection group and the untransfection group.wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group.The KFBs untransfected with wt-p53 gene served as control(untransfection group).After 48 hours of transfection,the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method,respectively.The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.Results All the KFBs from 2 groups expressed wt-P53 protein.But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group.At the same time of high expression of wt-P53 protein,the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group(P<0.05).Conclusion High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.