| HPLC法测定卵白蛋白脂质体中蛋白质含量 |
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| 引用本文： | 陈剑,黄泓,徐宇虹. HPLC法测定卵白蛋白脂质体中蛋白质含量[J]. 中国药房, 2011, 0(41): 3889-3891 |
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| 作者姓名： | 陈剑 黄泓 徐宇虹 |
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| 作者单位： | 上海交通大学药学院,上海市200240 |
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| 基金项目： | 国家自然科学基金资助项目（81001406） |
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| 摘 要： | 目的:建立测定卵白蛋白(OVA)脂质体中OVA含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX300SB-C8;流动相A为0.01%三氟乙酸水溶液,流动相B为0.01%三氟乙酸乙腈溶液,梯度洗脱,流速为0.8mL·min-1;检测波长为220nm;柱温为40℃。结果:OVA检测浓度线性范围为10～500μg.mL-(1r=0.9995);平均回收率为101.2%,RSD=0.74%(n=6)。结论:建立的测定方法灵敏度高、专属性强、简便快捷,可用于OVA脂质体中OVA的包封率以及含量的测定。
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| 关 键 词： | 脂质体 卵白蛋白 蛋白质 高效液相色谱法 含量 |
Content Determination of Protein in Ovalbumin Liposomes by HPLC |
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| CHEN Jian,HUANG Hong,XU Yu-hong. Content Determination of Protein in Ovalbumin Liposomes by HPLC[J]. China Pharmacy, 2011, 0(41): 3889-3891 |
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| Authors: | CHEN Jian HUANG Hong XU Yu-hong |
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| Affiliation: | (School of Pharmacy, Shanghai Jiaotong University, Shanghai 200420, China) |
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| Abstract: | OBJECTIVE： To establish a method for the content determination of ovalbumin （OVA） in OVA liposomes. METHODS：HPLC method was adopted. The separation was carried out on ZORBAX300SB-C8 column with mobile phase A consisted of 0.01% trifluoroacetic acid-water and mobile phase B consisted of 0.01% trifluoroacetic acid-acetonitrile （gradient elution） at the flow rate of 0.8 mL.min-a. The detection wavelength was set at 220 nm and column temperature was 40 ℃. RESULTS：The linear range of OVA was 10 to 500 μg·mL^-1 （r=0.999 5） with an average recovery of 101.2% （RSD=0.74%, n=6）. CONCLUSION： The method is found to be sensitive, specific and simple. It can be used for the content and encapsulation coefficency determination of OVA in OVA liposomes. |
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| Keywords: | Liposomes Ovalbumin Protein HPLC Content |
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