胰岛素样生长因子1及其受体在卵巢癌细胞增殖中的作用

目的探讨胰岛素样生长因子1(IGF1)及其受体(IGF1R)在卵巢癌HO8910PM细胞增殖中的作用。方法以不同浓度的IGF1作用于HO8910PM细胞24、48、72和96 h,通过CCK-8法检测细胞增殖活性;在脂质体介导下转染特异性IGF1R小干扰RNA(siRNA),通过实时PCR和Western blotting验证其在IGF1R mRNA和蛋白表达水平的抑制效果;于转染后48 h给予IGF1刺激,通过CCK-8法测定细胞增殖活性。结果IGF1作用48 h后可明显刺激细胞的增殖(P<0.05);转染IGF1R siRNA后48 h,IGF1R mRNA和蛋白表达均明显下调(P<0.05);转染IGF1R siRNA后48 h给予IGF1刺激,IGF1的促细胞增殖效应明显被抑制(P<0.05)。结论IGF1通过IGF1R途径促进HO8910PM细胞的增殖,IGF1R siRNA可以有效抑制该作用。

Effects of insulin-like growth factor 1 and its receptor on proliferation of ovarian cancer cells

Objective To investigate the effects of insulin-1ike growth factor 1(IGF1) and IGF1 receptor(IGF1R) on proliferation of HO8910PM cells of ovarian cancer. Methods The effects of IGF1 of different concentration on HO8910PM cells were observed by CCK-8 assay.Transfection of IGF1R siRNA by lipofectamine 2000 to silence IGF1R gene expression in HO8910PM,the silence effects were evaluated by real-time PCR and Western blotting.Forty-eight hours after transfection,IGF1 was added on HO8910PM cells to observe the effects on proliferation by CCK-8 assay. ResultsIncubation with IGF1 for 48 h significantly stimulated the proliferation response of HO8910PM cells(P<0.05).IGF1R mRNA and protein expression levels were significantly decreased by transfecting IGF1R siRNA for 48 h(P<0.05).After transfection of IGF1R siRNA for 48 h and incubation with IGF1,the stimulation of proliferation response was significantly down-regulated(P<0.05). Conclusion IGF1 stimulates the proliferation response of HO8910PM cells by IGF1R pathway.IGF1R siRNA may effectively down-regulate the effects.