基于水通道蛋白AQP1/AQP3的表达探讨石膏对急性软组织损伤大鼠模型的影响

目的研究生石膏和煅石膏对急性软组织损伤大鼠模型水通道蛋白AQP1、AQP3表达的影响,对石膏消肿的作用机制进行初步研究。方法采用砝码自由落体运动制备急性软组织损伤大鼠模型,造模成功后将动物随机分为模型组,阳性药组(双氯芬酸0.75 g/kg),煅石膏高、中、低剂量组(1.0、0.5、0.25 g/kg)、生石膏高、中、低剂量组(1.0、0.5、0.25 g/kg),另设空白对照组,每组10只。除模型组与空白对照组外,其余各组均给予相应药物,每天换药1次,连续7 d。采用Western blotting和免疫组化法检测各组大鼠损伤组织中AQP1/AQP3表达变化。结果与空白对照组比较,模型组大鼠损伤骨骼肌中AQP1/AQP3表达明显下降(P<0.01);与模型组比较,煅石膏高、中、低剂量组和阳性药组大鼠损伤骨骼肌中AQP1/AQP3表达明显增加(P<0.01),生石膏组无明显差异。结论石膏煅制后可能通过调节急性软组织损伤模型骨骼肌中AQP1/AQP3的表达从而发挥消肿的作用。

Exploring the Effects of Gypsum Fibrosum on Rat Model of Acute Soft Tissue Injury Based on the Expression of Aquaporin AQP1/AQP3

Objective To explore the effects of raw Gypsum Fibrosum and calcined Gypsum Fibrosum on Aquaporin AQP1 and AQP3 expression in acute soft tissue injury rats,and to preliminarily explore the swelling alleviating mechanism of Gypsum Fibrosum.Methods Acute soft tissue injury rat model was prepared by free-fall weight.After successful modeling,animals were randomly divided into a model group,a positive drug group(Diclofenac 0.75 g/kg),a calcined Gypsum Fibrosum high,medium and low dose group(1.0,0.5,0.25 g/kg),a raw Gypsum Fibrosum high,medium and low dose group(1.0,0.5,0.25 g/kg).Another blank control group was set,with 10 rats in each group.In addition to the model group and the blank control group,the other groups were given corresponding drugs,dressing once a day for 7 days.Western blotting and immunohistochemistry were used to detect the expression changes of AQP1/AQP3 in the rats injured tissue of each group.Results Compared with the blank control group,the expression of AQP1/AQP3 in skeletal muscle of model rats decreased significantly(P<0.01).Compared with the model group,the expression of AQP1/AQP3 in injured skeletal muscle of calcined Gypsum Fibrosum high,medium and low dose groups and positive drug group rats were significantly increased(P<0.01),and there was no significant difference in the raw Gypsum Fibrosum group.Conclusion Calcined Gypsum Fibrosum may play a role in eliminating swelling by regulating the expression of AQP1/AQP3 in skeletal muscle of acute soft tissue injury model.