散发性乳腺浸润性导管癌中BRCA1 表达及临床意义*

目的:研究散发性乳腺浸润性导管癌（invasive ductal carcinoma，IDC）（breastcancer 1 BRCA1）基因的表达及其与其临床病理特征之间的关系。方法:采用免疫组化SP三步法检测67例乳腺浸润性导管癌，53例乳腺腺病患者的石蜡标本BRCA1 的表达，从病理科的档案提取患者的年龄，肿物大小，淋巴结转移情况，ER，PR，Her-2 表达结果，分析BRCA1 的表达和患者的年龄，肿物大小，淋巴结转移情况及ER，PR，Her-2 表达的关系。结果:BRCA1蛋白在IDC 组织表达率56.7%（38/67），癌旁组织表达率80.0%（36/45），乳腺腺病组织表达率92.5%（49/53）。 阳性细胞定位除细胞核外，也见于胞浆。IDC 癌组织与癌旁组织、乳腺腺病组织比较，BRCA1 阳性表达率均下降，均有显著性差异（P<0.05）。 BRCA1 的表达与淋巴结转移呈负相关（r=-0.137，P<0.05），与ER、PR、Her-2 的表达均呈负相关（r 值分别为-0.439，-0.250，-0.363，P<0.05），与年龄、肿物大小无明显相关性（P>0.05）。 结论:BRCA1 蛋白可作为估计乳腺癌生物学行为和预后的潜在指标。 

BRCA1 Expression in Sporadic Invasive Ductal Carcinoma and Its Correlation with Other Biological Parameters

Objective:To investigate the expression of BRCA1 protein in sporadic invasive ductal carcino -ma and to analyze its relationship with other clinicopathologic parameters.Methods:The expression of BRCA 1 protein was detected by immunohistochemistry (IHC) with Streptavidin-Peroxidase (SP) method in 67 sporadic invasive ductal carcinoma tissue samples and 53adenosis tissue samples. The clinicopathologic pa-rameters including age of onset, tumor size, axillary nodal status, estrogen receptor, progesterone receptor and Her- 2 expression were obtained from pathological documents. The expression of BRCA 1 was compared between the sporadic invasive ductal carcinoma tissues and adenosis tissues. In sporadic invasive ductal car-cinoma, the correlation between BRCA1 expression and other clinicopathologic parameters mentioned above was further analyzed. Results: The positive expression rates of BRCA1 were56.7% (38/67) in breast cancer tissues and 92.5% (49/53) in adenosis tissues. In breast cancer tissues and cancer adjacent tussues, BRCA 1 was located in the cell nucleus as well as in the cytoplasm. The expression of BRCA1 was lower in breast can-cer tissues than in cancer adjacent tissues and adenosis tissues, with a significant difference ( P<0.05). The expression of BRCA1 was positively correlated with lymph node metastasis, and negatively correlated with the expression of estrogen receptor, progesterone receptor and Her- 2 expression ( P<0.05). No correlation was found between BRCA 1 expression and age of onset or tumor size ( P>0.05). Conclusion:BRCA 1 protein may be a potential marker for estimating the biological behavior and prognosis of breast cancer.