| PHA对单产和联产白细胞介素-2活性的影响 |
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| 引用本文： | 杨珊 刘鹏翰. PHA对单产和联产白细胞介素-2活性的影响[J]. 中国生化药物杂志, 1994, 15(1): 19-21 |
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| 作者姓名： | 杨珊 刘鹏翰 |
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| 作者单位： | 广西区血液中心!柳州545005 |
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| 摘 要： | 本文研究了用人外周血白细胞在细胞浓度为5×10~5个/ml、培养时间为24h,PHA加量对单独生产白细胞介素_2(单产)及联合生产a_干扰素、白细胞介素-2(联产)的影响.单产中,以25μg/mlPHA刺激量为宜;联产中以100μg/ml刺激量最好.联产比单产所得白细胞介素-2产量高10倍以上,且纯度高.
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| 关 键 词： | 白细胞介素-2 生产工艺 植物凝集素 |
Effect of PHA on the Activity of IL-2 Produced by Alone and United Generation Method |
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| Yang Shan,et alGuangxi Region Blood Center. Effect of PHA on the Activity of IL-2 Produced by Alone and United Generation Method[J]. Chinese Journal of Biochemical Pharmaceutics, 1994, 15(1): 19-21 |
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| Authors: | Yang Shan et alGuangxi Region Blood Center |
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| Affiliation: | Liuzhou 545005 |
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| Abstract: | This paper has studied the effect of PHA on the activity of Interleukin-2(IL-2) produced by the IL-2 alone and IL-2, α-Interferon(α-IFN) united method. To the former, when the human white blood cell concentration was 5×106 cell/ml, and culture time was 24 hours,25μ g/ml PHA was the best,and the latter, 100μg/ml needed. At the same PHA concentration, IL-2 produced by the united method was 10 times more than those of the alone method and the purity was higher. |
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| Keywords: | lnterleukin-2(IL-2) Generation method PHA |
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