肝素和氢化可的松对人肺癌抑瘤作用的体内实验研究

目的：研究肝素和氢化可的松的联合应用对人肺癌裸鼠皮下移植瘤生长抑制作用和对肿瘤血管生成、肿瘤细胞增殖及凋亡的影响，探讨肝素和氢化可的松联合应用抑制肿瘤血管生成的可能性。方法：建立人肺癌裸鼠皮下移植瘤模型，通过观察瘤体生长情况、免疫组化法测定微血管密度计数和肿瘤组织的增殖情况、电镜观察肿瘤组织凋亡情况和原位细胞凋亡检测，研究肝素和氢化可的松联合应用的抗血管生成和抑瘤作用。结果：肝素和氢化可的松治疗组瘤体生长较对照组明显受抑，且肿瘤组织微血管生成减少、增殖降低、凋亡增多。结论：肝素和氢化可的松联合应用具有明显的抗血管生成和抑瘤作用。

Experimental Study on Growth Inhibition Effect of Heparin andHydrocortisone to Human Lung Cancer

Objective:To study the growth inhibition effect of combination of heparin and hydrocortisone to xenograft lung tumors in nude mice,and to investigate the possibility of com-bined application of heparin and hydrocortisone in tumor angiogenesis inhibition.Methods:Af-ter establishing the model of xenograft lung tumor in nude mice,we treated them with these two drugs.The growth of the tumor and the modality of the tumor cells under optic and electron mi-croscope were observed,and expression of MVD and PCNA in the tumor were assayed.by im-munohistochemistry.Cell apoptosis was also detected by the method of TUNEL.Results:The growth of tumor treated with heparin and hydrocortisone was inhibited significantly as compared to the control.Less MVD and PCNA expression and more apoptotic cells were found in the tumor treated by heparin and hydrocortisone.Conclusion:The growth and angiogenesis of xenograft lung tumor in nude mice can be inhibited by combination application of heparin and hydrocortisone.