拳卷地钱不同提取部位的气相色谱-质谱分析比较和部分生物活性研究

基于拳卷地钱不同部位生物活性的不同 ,建立了采用气相色谱 -质谱 (GC/ MS)技术分析拳卷地钱不同提取部位的方法。拳卷地钱乙醇粗提物依次用石油醚和乙酸乙酯萃取 ,以 GC/ MS确定两部位挥发性成分的差异 ,并进行体外抗肝癌细胞试验。从石油醚部位共分离出 5 4个峰 ,鉴定了其中 2 5种物质 ,占总出峰面积的65 .0 %以上 ,在所分离的化合物中 ,以有机酸和酯类化合物为主 ;从乙酸乙酯部位分离出 2 3个峰 ,鉴定了其中 1 9种物质 ,占总出峰面积的 89.0 %以上 ,在所分离的化合物中 ,以苯并噻唑 (1 4.97% )和雪松醇 (2 0 .69% )为主。体外抗肝癌细胞试验 ,发现乙酸乙酯部位有明显抑制肝癌细胞的作用 ,并有剂量依赖关系 ;当质量浓度达 40 mg/ L时 ,对肝癌细胞有明显抑制作用。石油醚部位对肝癌细胞抑制作用不明显。

Comparison of Gas Chromatography-Mass Spectrometry Analysis in Different Extract Parts of Marchantia convoluta and Study on Partial Biologic Activity

The analysis method of different extract parts of Marchantia convoluta by gas chromatography-mass spectrometry(GC/MS) was established because of the different partial biologic activity in different parts of M. convoluta. The crude extracts of M. convoluta leaves was obtained after M. convoluta leaves were extracted with 80% ethanol and the extracts were concentrated under reduced pressure. Then the extract was partitioned using sequential extraction with organic solvents: petrol and ethyl acetate and n-butanol saturated with water. The petrol and ethyl acetate extracts were performed GC/MS analysis to identification the chemical constituents. Anti-liver cancer cells effect was studied. 54 peaks were separated and 25 compounds were identified by GC/MS from petrol extract. The main com-ponents were organic acid and ester (10). The other 7 kinds of terpenes and 8 kinds of terpenones were identified, too. 23 peaks were separated and 19 compounds were identified by GC/MS (reliability to standard MS >80%) from ethyl acetate extract. The main components were benzothianzole and cedrol (accounting for 14.97% and 20.69%). Only 4 kinds of organic acid and ester were identified from ethyl acetate extract. It was found that ethyl acetate extract induced cytotoxicity of liver cancer cells in a dose-dependent manner and 40mg/L of ethyl acetate extract can inhibited liver cancer cells evidently. Petrol extract had no effect on inhibiting liver cancer cells.